A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.
A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.6P
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Transcribed Image Text:A biochemist discovers and purifies a new enzyme, generating the purification table below.
Procedure
Total protein (mg)
20,000
5,000
4,000
Activity (units)
4,000,000
3,000,000
1,000,000
800,000
750,000
675,000
1. Crude extract
2. Precipitation (salt)
3. Precipitation (pH)
4. Ion-exchange chromatography
5. Affinity chromatography
6. Size-exclusion chromatography
From the information given in the table, calculate the specific activity of the enzyme after each
purification procedure.
b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the
greatest relative increase in purity)?
Which of the purification procedures is least effective?
d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is
now pure? What else could be done to estimate the purity of the enzyme preparation?
200
50
45
a.
C.
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