Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock solution.
Q: Based on the information given, what separation technique(s) is/are most likely feasible? Please…
A: The selective boiling and subsequent condensation of a part in a liquid mixture is referred to as…
Q: Below is a trace from an analytical gel-filtration column upon which a single sample containing a…
A: Gel filtration column is used in gel filtration chromatography (GFC) technique. GFC is also known as…
Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…
A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…
Q: Compare and contrast the following protein characterization techniques in terms of the principles…
A: A Physical method for the separation of compounds is called chromatography. In two…
Q: Gel-filtration chromatography is a useful method for removing salts, such as ammonium sulfate, from…
A: Gel filtration is a process during which molecules are separated based on their molecular size as…
Q: You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. For…
A: Atomic absorption spectrometry (AAS) is a simple, high-throughput, low-cost technique for analyzing…
Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…
A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…
Q: What are the mechanisms of samples separation work in Thin layer Chromatography?
A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…
Q: Which of the following chromatographic techniques has an attached ligand on the stationary phase?…
A: Chromatography is a laboratory technique that is used to separate components, or solutes, of a…
Q: Describe how you would prepare the required dilution series using the least amount of dilution water
A: Dilution is the process by which water is added to a solution or a chemical to make it less potent.…
Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…
Q: You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar,…
A: Media is providing the nutrients for growth of micro organism.
Q: What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or…
A: pH is the measure of the strength of H+ ion or Hydronium ions in solution. pOH is the measure…
Q: What is the basis for the separation of proteins by the following techniques? (a) gel-filtration…
A: Chromatography is a separation technique of chemical substances into individual components. and the…
Q: Briefly explain how you could use the color of the Coomassie assay sample solution to determine if…
A: Colorimetric protein tests, for example, the Coomassie blue G-250 color (Bradford) examines, are…
Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE
A: Gel filtration: with this method we could separate protein with respect to molecular weight in the…
Q: can you show how to calculate the amount of two components for the required volume of a buffer with…
A: Given Values: Concentration of Tris = 0.25 M Concentration of Glycine = 1.92 M Concentration of SDS…
Q: what type of gel must be used when the protein size is 2500 Da
A: A type of size exclusion chromatography in which the separation of components is based on the…
Q: give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete…
A: Introduction: A serial dilution is any dilution in which the concentration decreases by the same…
Q: 10mL of 1x solution from a 2x stock solution.
A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…
Q: You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a…
A: Dilution of Hydrogen peroxide (H2O2): Dilution of Hydrogen peroxide (H2O2), is carried out by adding…
Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…
A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…
Q: In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them
A: A type of gel electrophoresis (GE) that is used to quantify and separate desired protein molecules…
Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…
A: Chromatography is a biophysical method for separating compounds from a mixture. It works on the…
Q: What is native PAGE used for?
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: How many microliters of a 2.5X restriction enzyme buffer would be required if the total volume of…
A: Restriction enzyme buffer: Buffer selection is very important for obtaining the high activity of…
Q: A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation energy…
Q: Would a sample solution that has a high absorbance reading, have a low or a high concentration
A: Absorbance is a number that measures the diminishing of the transmitted radiant power in a solution.…
Q: Explain how to perform a serial dilution
A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…
Q: What will be the results after you reconstitued a cefixime 60 ml a powder for suspension and stand…
A: A coarse dispersion suspension is a system in which an internal phase(also called the solid…
Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time…
A: Ampicillin helps in retarding the growth of microorganisms by stopping their growth because it is an…
Q: briefly explain the principle behind this technique; gel-filtration chromatography
A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…
Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…
A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…
Q: Describe how the following technique work on protein purification? ammonium sulfate precipitation
A: Proteins are organic macromolecules that assist in maintaining the structural and functional…
Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…
A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.
Q: Why do you think surfactants denature proteins? Select all that apply.
A: Introduction: Protein are the most bountiful natural element of the living framework. They found in…
Q: Which of the following samples are expected to cause a decrease in absorbance over time when…
A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…
Q: When a mixture of various molecular sized dyes are run through a gel chromatography column, what is…
A: Chromatography is a process of separation of substances from mixtures based on their molecular size.…
Q: You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table.…
A: We are having the concentration of the ammonium nitrate and potassium nitrate at 1 litre or 1000 ml…
Q: Explain the principle of how electrospray ionization can be used for the analysis of large…
A: Mass spectrometry or MS is an analytical technique for determining the mass-to-charge ratio of ions.…
Q: List two situations when you would not be able to use the UV-Vis absorbance at 280 nm to determine a…
A: There are different ways of determining protein concentration. UV-Vis spectroscopy is one such…
Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…
A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…
Q: 5 L of 0.2 M dextrose solution from 5 M dextrose solution
A: Given Values: Stock solution of dextrose = 5 M Final solution of dextrose = 0.2 M Volume of the…
Q: You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?
A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…
Q: You want to use a serial dilution to make a 1/50 dilution. The first dilution you make is a 1/5…
A: Introduction Dilution is a process by which the concentrations of solute are lowered down in a…
Q: Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is…
A: Chromatography technique is used to separate mixtures. In this technique, there are 2 phases,…
Q: Search for the principles and the uses of the following methods for protein measurements and provide…
A: To measure total protein present in our body total protein lab test is to be done. The growth of…
Q: The chemical precipitation method can be used for ceramic nanoparticles production. * TRUE FALSE
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Step by step
Solved in 2 steps
- You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.
- In protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8You have a 100X stock of a buffer. You need 100 ml of 1X buffer. How much buffer solution do you use? How much water?How much of a 10x buffer should be added to 900ml of water to make a 1x solution?
- Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?How will you prepare a 5% BSA solution? Show all your steps.