Circle and label one place on the mechanism where covalent catalysis is occurring. Suggest an amino acid that could fill the role of residue “A” in the mechanism above and draw its structure at pH 7.4. Suggest an amino acid that could fill the role of residue “B” in the mechanism above and draw its structure at pH 7.4. Under standard conditions, this reaction is unfavorable (DG¢° = 23.8 kJ/mol). What conditions in the cell allow for the actual free energy change to be lower (DG » 0), making the reaction readily reversible? Explain your answer.

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  1. Consider the following mechanism:
    1. Circle and label one place on the mechanism where covalent catalysis is occurring.
    2. Suggest an amino acid that could fill the role of residue “A” in the mechanism above and draw its structure at pH 7.4.
    3. Suggest an amino acid that could fill the role of residue “B” in the mechanism above and draw its structure at pH 7.4.
    4. Under standard conditions, this reaction is unfavorable (DG¢° = 23.8 kJ/mol). What conditions in the cell allow for the actual free energy change to be lower (DG » 0), making the reaction readily reversible? Explain your answer.

*please help and explain as well as you can*

ÇH,OPO3- CH,OPO3-
н но
OH
H
ÓH H
Вох 1
CH,OPO-
| CH,OPO-
H 'CH,OPO?-
H+
H20
H
Lys-N:2c=0
H
H
-N+.
Lys-N*=C
*H-A-
-0-H :A-
:A-
HO?CH
:B-
н носн
HOCH
:B-
HªĊ-0-H
HC-0-H
-BH !
НСОН
-B:
-B:
H°COH
HÇOH
6CH,OPO?-
CH,OPO-
CH,OPO-
Вох 2
Вох 3
H.
CH,OPO3
C=0
НСОН
CH,OH
CH,OPO3-
H
Lys-N:
H
CH,OPO-
H. I
Lys-N*=¢
CH,OPO3-
H
H20
:A-
Lys-N-C
:A-
H-A-
HO-Ċ-H
:B-
H-B-
H-B-
-B:
— В:
-B-H
Transcribed Image Text:ÇH,OPO3- CH,OPO3- н но OH H ÓH H Вох 1 CH,OPO- | CH,OPO- H 'CH,OPO?- H+ H20 H Lys-N:2c=0 H H -N+. Lys-N*=C *H-A- -0-H :A- :A- HO?CH :B- н носн HOCH :B- HªĊ-0-H HC-0-H -BH ! НСОН -B: -B: H°COH HÇOH 6CH,OPO?- CH,OPO- CH,OPO- Вох 2 Вох 3 H. CH,OPO3 C=0 НСОН CH,OH CH,OPO3- H Lys-N: H CH,OPO- H. I Lys-N*=¢ CH,OPO3- H H20 :A- Lys-N-C :A- H-A- HO-Ċ-H :B- H-B- H-B- -B: — В: -B-H
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