Answered: A certain polypeptide was treated with…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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1. A certain polypeptide was treated with trypsin and yielded the following Fragments: Leu-Glu Gly-Tyr-Asn-Arg Gln-Ala-Phe-Val-Lys The same polypeptide was treated with chymotrypsin and yielded the following fragments: Gln-Ala-Phe Asn-Arg-Leu-Glu Val-Lys-Gly-Tyr What is the amino acid sequence of this polypeptide? Instructions Make use of the table below to determine the sequence of the mystery protein.

Reagent
Cleavage Points
Trypsin
Lys, Arg (C)
Submaxillary protease
Arg (C)
Chymotrypsin
Phe, Trp, Tyr (C)
2/4
Staphylococcus aureus
Asp, Glu (C)
Asp-N-protease
Asp, Glu (N)
Pepsin
Leu, Phe, Trp, Tyr (N)
Endoproteinase Lys C
Lys (C)
Cyanogen bromide
Met (C)
Carboxypeptidase A
Works on all residues (C) except Pro, Arg, Lys,
Asp, and Glu
Carboxypeptidase B
Arg, Lys (C)

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