A general transduction experiment was conducted to determine the gene order of trp, pyrF and qts loci on the bacterial chromosome. The Donor genotype is: trp+pyr+qts The Recipient genotype is: trp pyr qts+ The selected marker in this transduction experiment is trp+. Unselected Markers Number of colonies pyr+qts+ 22 pyr+qts 10 pyr qts+ 68 pyr qts 0 What is the full genotype of each of the 22 colonies for the 3 loci? What is the gene order of the 3 loci on the donor chromosome? How is co-transduction relevant to determining the answer to part b? Why is the name of the viral vector not given to help you answer this question?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A general transduction experiment was conducted to determine the gene order of trp, pyrF and qts loci on the bacterial chromosome.
The Donor genotype is: trp+pyr+qts
The Recipient genotype is: trp pyr qts+
The selected marker in this transduction experiment is trp+.
Unselected Markers Number of colonies
pyr+qts+ 22
pyr+qts 10
pyr qts+ 68
pyr qts 0
- What is the full genotype of each of the 22 colonies for the 3 loci?
- What is the gene order of the 3 loci on the donor chromosome?
- How is co-transduction relevant to determining the answer to part b?
- Why is the name of the viral vector not given to help you answer this question?
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