A microbiologist sets up a serial dilution to analyze bacteria in raw milk. Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3?
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- A microbiologist sets up a serial dilution to analyze bacteria in raw milk.
Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water
Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water
Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water
What is the dilution of raw milk in Tube 3?
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- You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile saline. One mL from the first bottle is transferred into a second bottle of saline containing 9 mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline. You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies, while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking water? This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.The below photograph shows a Gram-stained slide viewed using a light microscope set at brightfield of a clinical sample from a patient with symptoms suggesting an infection. The slide was viewed with x100 objective. What could be the most likely cause of infection? Gram positive bacteria, either rods or cocci Gram positive rod shaped bacteria or yeast Gram negative rod or cocci shaped bacteria Gram negative bacteria, either rods or cocciThe culture primarily contains nutrients such as sugars and a gel (agar). Why don’t viruses grow in a culture like bacteria do? Explain.
- Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 9) What makes the bacteria Gram negative?10) Approximately how long would it take you to finish the entire procedureThe students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
- A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Streptococcus mutans? Cells are arranged in chains. Secretes acids produced from metabolism of sucrose. Cells are cocci. Can grow in biofilms. Cells stain Gram negative.A scientist transfers 0.1 mL phage stock to a tube containing 9.9 mL tryptic soy broth. She then transfers 0.1 mL from that tube to a second tube containing 9.9 mL. She then plated 0.1 mL of the content in the second tube. The total fold of dilution is: a) 100,000x b) 1000x c) 100x d) 10,000xA microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Helicobacter pylori? Cells have flagella when viewed by flagellar staining. Secretes acids produced from metabolism of sucrose. Cells are curved rods. Produces and secretes urease enzyme. Cells stain Gram negative.
- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusPlease do not give solution in image format thanku 6. Legionnaires' disease is a type of pneumonia caused by bacteria. It is usually acquired through the __________. The bacteria can come from hot tubs, showers, or air conditioning units in large buildings. to. Contaminated water suction b. Unsanitized bedding c. soak in drinking water d. Contaminated water intake 7. What is the common name for sections of pipes in buildings filled with sewage in order to prevent sewage gases and bad odors from entering the building? to. traps b. sanitary pipes c. Gas flow preventer d. risersYou have a culture of bacteria with an unknown number of bacteria in it. You have at your disposal 10 1-mL pipettes, 3 dilution bottles that hold up to 100mL of volume, 3 test tubes that hold up to 10mL of volume, a graduated cylinder, an unlimited supply of distilled water. Explain how you would set up a 1: 10,000,000 dilution of your culture.