You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile saline. One mL from the first bottle is transferred into a second bottle of saline containing 9 mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline. You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies, while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking water? This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.

You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile saline. One mL from the first bottle is transferred into a second bottle of saline containing 9 mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline. You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies, while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking water? This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 1.1P

See similar textbooks

Similar questions

You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?
From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later, this suspension is analyzed in the Petroff-Hausser chamber, It is determined that the total number of bacteria found is 53 bacteria per square, if the chamber has 25 squares, how many bacteria are found in the chamber? initial juice sample?
1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is analyzed in the Petroff-Hausser chamber, determines that the total number of bacteria found is 44 bacteria per square, if the chamber has 25 squares, how many bacteria are in the chamber? initial juice sample?
Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.
If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40 bacteria what would be the dilution scheme of using 4 dilutions
Do you think standard plate counts are very accurate? Why or why not? When doing serial dilutions, why is it necessary to plate more than one dilution? You left a carton of orange juice on your counter for 3 days. When you taste it, it is very bubbly (as though it was carbonated), and it tastes more bitter than usual. (Note: acids taste bitter). What do you think could have happened? (Note: you can’t just say that bacteria grew—you must explain how the growth of the bacteria resulted in the changes in the orange juice.) Based on what you now know about the presence of bacteria on chicken and beef, why is it a good idea to use separate chopping boards for meat and for vegetables? Please help me thank you
What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200
You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you plated 0.15mL if each dilution onto each of 3 plates of nutrient agar. After incubation, the colonies were way too numerous to counton the plates prepared from the 10-7 dilution. The plates from the 10-8 dilution had 355, 360, and 350 colonies. The platesfrom the 10-9 dilution had 115, 117, and 118 colonies. Determine the concentration of colony forming units (cfu/mL) inthe original bacterial suspension
A bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300. Determine the concentration of cells after EACH dilution. Concentration after the 1st dilution = 6.3 x 107 cells/mL x 1/5 = 12.6 x 106 cells/mL Concentration after the 2nd dilution = 12.6 x 106 cells/mL x 1/300 = 42000 cells/mL
In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25 colonies the next day. What was the concentration of cells in the original culture? A) 25 cells/ml B) 0.000025 cells/ml C) 25,000 cells/ml D) 26 cells/ml E) 0.025 cells/ml
You decide to sample the bacteria growing on several items in a laboratory space. You follow aseptic techniques and use applicator swabs dipped in a sterible broth solution to sample from the floor, the sink, and your shoe. You spread each sample on separate labeled agar plates for incubation. a. You want to be sure that the sterile solution you used for sampling was not contaminated. How would you set up a negative control to test this? b. What result for your negative control would indicate that something is contaminated?