A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?

A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 17SST

See similar textbooks

Similar questions

An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?
An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?
A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated. After incubation, 150 colonies appeared. what is the CFU/mL in the original culutre?
In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are counted. Answer the question below. there were _____cells in the 1 mL of culture that was spread on the plate. If the culture was diluted 1:100 prior to plating, how many cells were there per mL in the original culture? _________
one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?
You plate 100 uL of a bacterial culture on an LBA plate and incubate for one day at 37 degrees Celsius. You count 100 colonies (colony forming units). What is the concentration of cells you plated (represented in units of CFU/mL)? Hint: look at the units carefully. I put 10000 last time and it was wrong!! that is all the information given... 5 cfu/mL b. 1,000 cfu/mL c. 500 cfu/mL d. 1 cfu/mL e. 10,000 cfu/mL
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies Calculate the CFU/mL for this juice. Show formula used and include units
Idenitfy the statements whether they are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.
A cell is 25 μm wide when viewed at 1,000× magnification. Thismeasurement can also be written properly asa. 25 mm. c. 0.025 mm.b. 25,000 mm. d. 2.5 mm.
A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how many CFU/ml are in the urine if 158 colonies grow?