A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate. What do you expect to grow on the Chloramphenicol Plate? Select one or more: a. E. coli with no plasmid vector b. Impossible to determine with the provided information c. Nothing will grow d. E. coli with the gene of interest in your plasmid vector e. E. coli with the original plasmid vector

A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate. What do you expect to grow on the Chloramphenicol Plate? Select one or more: a. E. coli with no plasmid vector b. Impossible to determine with the provided information c. Nothing will grow d. E. coli with the gene of interest in your plasmid vector e. E. coli with the original plasmid vector

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 11QP: Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA...

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In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site inside of the strR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. What do you expect to grow on the master plate without any antibiotics? What do you expect to grow on the streptomycin plate? What do you expect to grow on the kanamycin plate? How do you determine which colonies contain your gene of interest? As mentioned above, there is a restriction site found within the strR Please draw or write out two different examples of what restriction sites may look like. These should be short…
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site within the strR gene that you are hoping to move your gene of interest into. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. 1. Using those same pieces of short DNA that you created above, show me what one of them may look like if it was cut with a blunt end restriction endonuclease. With the other example, show me what it would look like if it was cut with a sticky end restriction endonuclease. 2. Why do restriction endonucleases exist in bacterial cells? What must they do to protect their own DNA?
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site within the strR gene that you are hoping to move your gene of interest into. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. 1. What do you expect to grow on the master plate without any antibiotics? 2. What do you expect to grow on the streptomycin plate? 3. What do you expect to grow on the kanamycin plate?
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site within the strR gene that you are hoping to move your gene of interest into. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. 1. How will you determine which colonies contain your gene of interest? Be specific about what you will be screening for. 2. As mentioned above, there is a restriction site found within the strR gene. Please draw or write out two different examples of what restriction sites may look like. These should be short pieces of double stranded DNA.
A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate. What do you expect to grow on the Master Plate? A. E. coli with the gene of interest in your plasmid vector B. Impossible to tell with the provided information C. Nothing will grow D. E. coli with the original plasmid vector E. E. coli with no plasmid vector
When a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid, how many bands can be visualized on an agarose gel? a)1 b)2 c)5 d)3
A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?
Now that you’ve isolated the gene and made lots of copies, you need to insert the gene into something you can manipulate and move into your lab strain of E. coli. You have access to the following vectors, either pBR322 or pUC19 (look them up here to see a map https://www.neb.com/products/dna-plasmids-and-substrates Please note the restriction sites in BOLD appear only once in the plasmid). You may use either vector. What is a vector? Which vector did you choose? Explain why you made that choice. What enzyme(s) will you use to place your fragment into the vector? Explain why you chose these enzymes. How will you move this construct into coli? What phenotype will tell you if the coli have taken up the plasmid? What phenotype will tell you if the coli have taken up the plasmid with the gasP gene?
true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme cuts between the second A and the C, this will produce a "sticky end," which is useful for cloning into a plasmid vector.
A biotech lab technician is working with a mutated form of a particular bacterial gene and wants to obtain large amounts of plasmid DNA that contains this gene. In this situation, the gene should be in which of the following type of vector (to introduce into E. coli)? Expression vector. Shuttle vector. F plasmid. Cloning vector.
You have a recombinant plasmid containing a vector and a segment of foreign DNA, both equal sizes. Draw a picture of this recombinant plasmid labeling foreign and vector regions. Where the foreign DNA meets the vector, there is a cut site for restriction enzyme ABC1. When the recombinant plasmid is cut by ABC1, how many fragments do you expect to be produced? Identify these fragments.