A sample of enzyme involved in alcohol metabolism, extracted and purified extensively from yeast cultures, was loaded onto a gel filtration column for molecular weight approximation. A set of protein standards with known molecular weight was eluted separately through the same column and suitable buffer following the elution of blue dextran for void volume determination. The following table summarizes the data obtained from the experiment: Elution flow rate 1 mL/min Sample collector setting Continuous; Sequential Container change rate 1 tube/min Sample/Standard eluted Test tube No/s. MW (Da) Blue dextran 4 2,000,000 Bovine thyroglobulin 11 669,000 Horse spleen apoferritin 14 443,000 Beta-amylase from sweet potato 21 200,000 Bovine serum albumin 27 66,000 Alcohol metabolism enzyme 19 ? 1. Process the given raw data in a tabular format of properly identified data points ready for calibration curve plotting. 2. Construct the calibration curve using MS Excel for use in MW determination. Note that the axes must be properly labeled and the trend line must be shown. 3. Determine the r?, slope, and y-intercept of the calibration curve. 4. Estimate the MW of the alcohol metabolism enzyme. Show your complete solution.

A sample of enzyme involved in alcohol metabolism, extracted and purified extensively from yeast cultures, was loaded onto a gel filtration column for molecular weight approximation. A set of protein standards with known molecular weight was eluted separately through the same column and suitable buffer following the elution of blue dextran for void volume determination. The following table summarizes the data obtained from the experiment: Elution flow rate 1 mL/min Sample collector setting Continuous; Sequential Container change rate 1 tube/min Sample/Standard eluted Test tube No/s. MW (Da) Blue dextran 4 2,000,000 Bovine thyroglobulin 11 669,000 Horse spleen apoferritin 14 443,000 Beta-amylase from sweet potato 21 200,000 Bovine serum albumin 27 66,000 Alcohol metabolism enzyme 19 ? 1. Process the given raw data in a tabular format of properly identified data points ready for calibration curve plotting. 2. Construct the calibration curve using MS Excel for use in MW determination. Note that the axes must be properly labeled and the trend line must be shown. 3. Determine the r?, slope, and y-intercept of the calibration curve. 4. Estimate the MW of the alcohol metabolism enzyme. Show your complete solution.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter12: Spectrochemical Methods

Section: Chapter Questions

Problem 7P

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