A series of auxotrophic mutants were isolated in Neurospora. Examinationof fungi containing these mutations revealed that they grew on minimalmedium to which various compounds (A, B, C, D) were added; growthresponses to each of the four compounds are presented in the followingtable. Give the order of compounds A, B, C, and D in a biochemicalpathway. Outline a biochemical pathway that includes these fourcompounds and indicate which step in the pathway is affected by each ofthe mutations.CompoundMutation numberА в СAD134276987773772146333123+ + I +

A biochemical pathway is a series of different chemical reactions that results in the formation of a…

Answered: A series of auxotrophic mutants were…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

See similar textbooks

Similar questions

A number of auxotrophic mutant strains were isolated from wild-type haploid Neurospora crassa. These strains responded to the addition of certain nutritional supplements to minimal culture medium either by growth (+) or no growth (0) The data from this experiment are presented in the table below. Diagram a biochemical pathway, complete with positions of intermediates, that is consistent with the data. Indicate where in the pathway each mutant strain is blocked.
The intermediates A, B, C, D, E, and F all occur inthe same biochemical pathway. G is the product of thepathway, and mutants 1 through 7 are all G−, meaningthat they cannot produce substance G. The followingtable shows which intermediates will promote growthin each of the mutants. Arrange the intermediates inorder of their occurrence in the pathway, and indicatethe step in the pathway at which each mutant strain isblocked. A + in the table indicates that the strain willgrow if given that substance, an O means lack of growth.SupplementsMutant A B C D E F G1 + + + + + O +2 O O O O O O +3 O + + O + O +4 O + O O + O +5 + + + O + O +6 + + + + + + +7 O O O O + O +
Assume that a series of compounds has been discovered in Neurospora. Compounds A–F appear to be intermediates in a biochemical pathway. Conversion of one intermediate to the next is controlled by enzymes that are encoded by genes. Several mutations in these genes have been identified and Neurospora strains 1–4 each contain a single mutation. Strains 1–4 are grown on minimal media supplemented with one of the compounds A–F. The ability of each strain to grow when supplemented with different compounds is shown in the table (+ = growth; o = no growth). Which biochemical pathway fits the data presented? Media Supplement Strain A B C D E F 1 o o o + + + 2 o o o o + + 3 o o o o + o 4 o o + + + + A) A → B → C → D → E → F B) A → B → C → F → D → E C) F → B → C → D → A → E D) A → B → C → D → F → E E) A → B → F → E → C → D
When present on the leaves of plants, the bacterium Pseudomonas syringae can promote frost damage to plants. Mutant strains, lacking the “ice” gene, have been applied to plants to try and protect the plants from wild-type P. syringae-induced frost. Assume that I am telling the truth that wild-type P. syringae nucleates ice formation at -2 °C, and provide an explanation as to why application of this altered (mutant) bacterium to plants might be a beneficial agricultural strategy in areas where morning lows occasionally dip down to 28-30 °F.
How can the biotranformation of l-ctronellal to l-citronellol using free and immobilized cells of Rhodotorula minuta be altered to improve yield of citronellol?
For the study of alanine production by a recombinant strain of E. coli, cultivation was carried out in a benchtop bioreactor with 4.5 L of culture medium, using glucose as a limiting substrate. During the cultivation, there was no lag phase and the cells showed exponential growth for 5 hours. The following table presents the results of the analysis of ammonia and glucose consumption, and alanine accumulation throughout the cultivation. Knowing that 500 mL of a cell suspension at a concentration of 5.0 g/L (inoculum) was added to the 4.5 L of medium in the reactor and that the YX/NH3 previously determined was 7.5, calculate:a) the maximum specific growth rateb) YX/S and YP/S yield factorsc) How long would it take to reach Cx = 30 g/L if the cells continued with the exponential growth profile until the end of the culture (without nutrient deprivation or any type of inhibition)?d) Describe how the mathematical treatment of the data should be done to determine the type of product formation…
Why are regulatory mutants used to increase the production of glutamic acid by Corynebacterium glutamicum?
A conjugate was incubated with a mixture of lysosomal enzymes and no drug release was observed in these conditions. The same conjugate was tested for antitumor activity in vivo and an anticancer effect was observed. Taking into account the information provided above, hypothesize a possible mechanism of action for this conjugate.
For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
There is a group of six or so genes, called luxlux genes, whose gene products are necessary for light formation. Given that these bacterial genes are regulated together, propose a hypothesis for how the genes are organized and regulated. Match the terms in the left column to the appropriate blanks in the sentences on the right. Terms can be used once, more than once, or not at all. Terms: metaboliozed, density, quorum sensing, state, signal amplification, inhibit, activate, detected, secrete, transcrption initiation complex, consume, operon Bacterial cells blank molecules that can be blank by other bacterial cells. Sensing the concentration of such signalling molecules allows bacteria to monitor the local blank of cells, a phenomenon called blank. There is a group of genes in the genome of the described bacterium, called luxgenes, which are organized in an blank. Reaching a certain concentration, molecules involved in this process blank the luxgenes. As a result, the…
Resistance to many penicillins is the result of cell wall mutations in a variety of bacteria. True or false?
You are tasked with cultivating the following microorganisms:A recombinant strain of E. coliA wild strain of Streptomyces clavuligerus• Suggest a storage method for each microorganism and justify your choice. You have at your disposal a refrigerator and an ultrafreezer, in addition to materials and conditions for handling microorganisms.• Propose a culture medium for each (choose a complex medium for one organism and a synthetic medium for the other)As a complement of nutrients, they must be indicated on the following scale: +++ : above 5 g/L++ : from 1 to 5 g/L+ : below 1 g/L• Describe the procedure for preparing and sterilizing the components of the culture media for a volume of 5.0 L• Make a bench process selection scheme, up to the step of selecting a benchtop reactor 5 L.

SEE MORE QUESTIONS

Recommended textbooks for you

Biology: The Dynamic Science (MindTap Course List)

Biology

ISBN:

9781305389892

Author:

Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:

Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Biology: The Dynamic Science (MindTap Course List)

Biology

ISBN:

9781305389892

Author:

Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:

Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

SEE MORE TEXTBOOKS