For her undergraduate project, Jessica studied an enzyme that catalyzes the reaction A = B. For substrate A, she determined that Km = 2.5 µM and kcat = 35 min-. Jessica graduated and her project has been passed on to you. Unfortunately, Jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. Your mentor suggests that you try to back calculate some of the missing concentration values. Assume that the enzyme follows Michaelis- Menten kinetics. In experiment A, where [A] = 5 mM, she found that the initial velocity, Vo, was 454 nM min-'. What was the [E] in experiment A? [E] = 12.98 nM %3D

Q: Using lysozyme as an example, what can an enzyme’s structure reveal about its catalytic mechanism?

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Q: You have performed protein purification on your new favorite enzyme using a protocol which involves…

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Q: Consider the following data set of enzymes A, BC and D which catalyze the same reaction. Enzyme A km…

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Q: Why is it somewhat misleading to study biochemical pathways separately?

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Q: How can we predict the effect of mutations on the catalytic efficiency of enzyme?

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Q: For an enzyme that obeys Michaelis-Menten kinetics, in order for the reaction velocity (v) to be 80%…

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Q: What class does your HindIII enzyme belong?

A: HindIII is a restriction endonuclease (RE).

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Q: Imagine you have an enzyme-catalyzed reaction where the enzyme has a Km of 2 mM and a Vmax of 10…

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Q: What is the effect of denaturing an enzyme?

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Q: Graphically explain the term saturation of the enzyme? Why is the rate of an enzymecatalyzed…

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Q: What effect does the optimum pH and temperature have on enzyme function? Briefly explain with an…

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Q: Why is the regulation of biochemical processes important?List three reasons

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Q: Compounds I, II, and III are in the following biochemical pathway: precursor compound I enzyme A…

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Q: You are studying a bacterial metabolic pathway that results in the synthesis of a product, vitalin,…

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Q: Why are buffers important in biochemical experiments?

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Q: A TAMU undergraduate biochemistry student is tasked with characterizing a new enzyme. After devising…

A:

Q: To measure Kcat and Km, we use an enzyme assay and titration the substrate. How is this done?

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Q: Imagine an allosteric enzyme is modified so that it exists 100% in the R state or 100% in the T…

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Q: Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340…

A:

Q: If you did not use a saturating concentration of pNPP to establish your initial calibration curve,…

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Q: Which of the following is NOT true about enzymes     A. they do not alter reaction equilibria…

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Q: Suppose we have 2 proteins A and B with the following kcat and Km values. Prot. A 5.4x10 50 Prot. B…

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Q: Is it possible to envision models for the behavior of allosteric enzymes other than the ones that we…

A: Yes, it is possible to envision models for the behaviour of allosteric enzymes other than the ones…

Q: One of the important features of enzymes is that they stabilize transition state of the reaction…

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Q: With a branched biochemical pathway, why would it be important for a cell to shut down the first…

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Q: Is the term KM used with allosteric enzymes? What aboutcompetitive and noncompetitive inhibition?…

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Q: If the data from an enzyme experiment is plotted as a Lineweaver-Burk plot, and the Vmax is 0.02…

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Q: 1/V, min/umol 5.50 5.00 4.50 4.00 3.50 answer. 3.00 2.50 2.00 0.00 y = 0.9474x + 2.6649 0.50 1.00…

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Q: Suppose we have 2 proteins A and B with the following kcat and Km values. kcat (s) Km (uM) Prot. A…

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Q: Is the following statement correct or incorrect? It is only possible to employ enzymes that are…

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A:

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Q: Why is it important to know the Km and Vmax values of an enzyme

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Q: what is meant by S195? D102? H57?

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Q: at is the difference between enzyme limited and a substrate limited reaction?

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Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.