(a) Wakil's pioneering studies on fatty acid synthesis included the crucial obs is required for the synthesis of palmitoyl-CoA. He was surprised to find that carbonate could sustain palmitate synthesis; that is, there was no correlation picarbonate required and the amount of palmitate produced. Later he also ob: bicarbonate is not incorporated into palmitate. Explain these observation

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3.
(a) Wakil's pioneering studies on fatty acid synthesis included the crucial observation that
bicarbonate is required for the synthesis of palmitoyl-CoA. He was surprised to find that very low
levels of bicarbonate could sustain palmitate synthesis; that is, there was no correlation between the
amount of bicarbonate required and the amount of palmitate produced. Later he also observed that
14C-labeled bicarbonate is not incorporated into palmitate. Explain these observation
(b) The condensation reaction catalyzed by B-ketoacyl-ACP synthase generates a 4-carbon unit by
combining a 2-carbon unit with a 3-carbon unit and loss of CO2. Write with structural formulas how
this occurs. What is the thermodynamic advantage of this way of generating the 4-carbon unit over
combining two 2-carbon units as occurs fatty acid oxidation or ketone body reactions"
(c) Decide where labeled carbon atoms will be found in palmitate if (i) (3-1“C)malonyl-SCOA or (ii) (2-
14C)malonyl-SCOA are used.
Transcribed Image Text:3. (a) Wakil's pioneering studies on fatty acid synthesis included the crucial observation that bicarbonate is required for the synthesis of palmitoyl-CoA. He was surprised to find that very low levels of bicarbonate could sustain palmitate synthesis; that is, there was no correlation between the amount of bicarbonate required and the amount of palmitate produced. Later he also observed that 14C-labeled bicarbonate is not incorporated into palmitate. Explain these observation (b) The condensation reaction catalyzed by B-ketoacyl-ACP synthase generates a 4-carbon unit by combining a 2-carbon unit with a 3-carbon unit and loss of CO2. Write with structural formulas how this occurs. What is the thermodynamic advantage of this way of generating the 4-carbon unit over combining two 2-carbon units as occurs fatty acid oxidation or ketone body reactions" (c) Decide where labeled carbon atoms will be found in palmitate if (i) (3-1“C)malonyl-SCOA or (ii) (2- 14C)malonyl-SCOA are used.
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