a. Calculate the Km and Vmax of the reaction b. A competitive inhibitor is added to the reaction at a concentration of 5 μM, determine the new Vmax of the reaction and what happened to the Km value.

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter15: Recirculating Systems
Section: Chapter Questions
Problem 3E
icon
Related questions
Question

The kinetic data of an enzymatic reaction shown in the following table:

a. Calculate the Km and Vmax of the reaction
b. A competitive inhibitor is added to the reaction at a concentration of 5 μM, determine the new Vmax of the reaction and what happened to the Km value.

 

[S] in µM
Vo in μΜ/min
2
2.9
3.8
4
4.4
5
5.0
5.4
7
5.8
8
6.2
9.
6.4
10
6.7
Transcribed Image Text:[S] in µM Vo in μΜ/min 2 2.9 3.8 4 4.4 5 5.0 5.4 7 5.8 8 6.2 9. 6.4 10 6.7
Expert Solution
steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Recommended textbooks for you
Aquaculture Science
Aquaculture Science
Biology
ISBN:
9781133558347
Author:
Parker
Publisher:
Cengage
Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:
9781305143531
Author:
CURREN
Publisher:
Cengage
Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:
9781133893943
Author:
ESTRIDGE
Publisher:
Cengage
Principles Of Radiographic Imaging: An Art And A …
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning
Principles Of Pharmacology Med Assist
Principles Of Pharmacology Med Assist
Biology
ISBN:
9781337512442
Author:
RICE
Publisher:
Cengage