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- Recrystallized 2-naphthol compared to literature values • Format the table and the descriptive paragraphs. Full comparisons of peaks are to be made with reference to the SDBS database for all three compounds.In an IR spectroscopy of a recrystallized salicylic acid Discuss other chemical analysis methods that could be used to further verify that the compound you obtained is what you desired.Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?
- How would you interpret this IR spectroscopy of 9-fluorenone? Some background information is that this was a lab to separate fluorene and 9-fluorenone. It should be noted that other solids and solvents used during the column chromatography was alumina, sand, petroleum ether, and dichloromethane. The original compound was 0.05 g but it yielded 0.03 g 9-fluorenone. There may or may not be impurities.a) How does mass spectrometry identify organic compounds? b) Describe the use and function of Quadrupole Analyzers ? c) One of the ionization sources for atomic mass spectrometry (MS) is Inductively Coupled Plasma (ICP). Explain the need for an interface in the ICPMS instrument and how can it be achieved.An unknown mixture was analyzed with TLC on a silica gel plate. Development gave two spots with Rf values of 1.0 and 0.0 respectively.a) Can you say for sure how many compounds are present in the mixture? If so, how many are there? Explain. b) If not, what additional TLC experiment(s) might be performed to determine how many components are present? Explain.
- QUALITATIVE DETECTION OF ORGANIC COMPOUNDS Would you say that all organic compounds give evidence of containing carbon when they are burned or heated? Compare the color of acetanilide mixture before and after heating. What material was responsible for the color of the residue in the combustion tube after heating ? Is its formation a result of oxidation or reduction? Explain.how do you call the analysis technique done by identifying the fragments corresponding to each spectra. Index of Hydrogen deficiency Rule of 13 Fragmentation Nitrogen RuleExplain results for nitrous acid-observation, hinsberg test-observation and infrared and name unknown.
- A 50.0 mL juice extract is colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. Calculate the concentration of the reducing sugar in the 50.0 mL sample in mg/mL. Apply Beer-Lambert’s law: A=bc.In the IR Spectroscopy, describe the sample preparation techniques for: 1) solid, 2) liquid, 3) gas samplesQuestion: - Stray light (spectral purity) gives potentially rise to incorrect absorbance measurements. a) Is this more likely to occur at low analyte concentrations or high analyte concentrations? Explain your answer. b) When stray light is present, will it cause you to determine a lower analyte concentration, a higher one or does it have no impact on the determination of the analyte concentration?