a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: In S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x + 8.01 mg species I solution S = x = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: In S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x + 8.01 mg species I solution S = x = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter5: Structure And Function Of Plasma Membranes

Section: Chapter Questions

Problem 7RQ: A scientist compares the plasma membrane composition of an animal from the Mediterranean coast with...

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