Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer. Dissolution of protein into pH 6.4 coating buffer is the first step of an ELISA. I. HEPES II. Phosphoric acid III. TRIS IV. Acetic acid V. Citric acid VI. Carbonic acid

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 4.6P

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Match the common laboratory scenarios to the correct buffer required using the guide below.

Molecule	pKa Value(s)
Acetic acid	4.8
Carbonic acid	6.1, 10.2
Citric acid	3.1, 4.8, 5.4
HEPES	7.5
Phosphoric acid	2.1, 7.1, 12.3
TRIS	8.3

	Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments.
	Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer.
	Dissolution of lysozyme requires pH 3 buffer to maintain activity.
	Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.
	Dissolution of protein into pH 6.4 coating buffer is the first step of an ELISA.

I.	HEPES
II.	Phosphoric acid
III.	TRIS
IV.	Acetic acid
V.	Citric acid
VI.	Carbonic acid

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