DISCUSS THE TWO GENERAL METHODS EMPLOYED IN OBTAINING FATS AND OILS FROM SOME OF THE USUAL SOURCES EXPRESSION SOLVENT EXTRACTION COMBINATION OF THE TWO: A AND
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DISCUSS THE TWO GENERAL METHODS EMPLOYED IN OBTAINING FATS AND OILS FROM SOME OF THE USUAL SOURCES
- EXPRESSION
- SOLVENT EXTRACTION
- COMBINATION OF THE TWO: A AND
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- Many culture media produce colorful solutions due to the presence of which are water soluble organic compounds that change color whenever there is a change in pH. Enumerate the indicators used, indicate the tests where they are used and give their specific reactions.Many culture media produce colorful solution due to the presence of which are water soluble organic compounds that change color whenever there is a change in pH. Enumerate the indicators used, indicate the tests where they are used and give their specific reactions.Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____
- Assumed that you have isolated the protein cytochrome C from a cell lysate. Discuss the advantages and disadvantages of the following protein assays. a. Absorbance at 215 nm. b. Absorbance at 280 nm. c. The Bradford assay.For the determination of total lipid content in food samples which matrix effects are expected to be significant, suggest an appropriate method with reasons and outline the corresponding procedures.In ion-exchange chromatography, amino acids are separated on the basis of electro-static interactions and hydrophobic interactions with the resin. The chemical structure of the resin pol-ymer with a negatively charged sulfonic acid group is shown to the right. An elution profile for the aminoacids using this resin is given below. Based on the elution profile above, explain the following:
- Suppose a positive Barfoed's test was obtained with sucrose solution suggest and 2-3 sentence explanationPrepare a complete analysis procedure using KMnO4 as teh oxidant instead of K2CrO7 in the redox analysis of iron. Include sample and solution preparation, approximate weights of samples and reagents, procedure and chemical reactions. In particular, what is the purpose of the Zimmermann-Reinhardt reagent?Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L
- Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include chemical reactions if necessary.In a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.Mention assays other than biuret and lowry assays that are used to detect the presence of proteins. Talk about the principle, the advantages, and the disadvantages.