Background and Procedure: The chemical bromothymol blue is an indicator that appears blue in an alkaline (base) medium and yellow- green in an acid medium. CO2 has been added to the bromothymol blue. Carbon dioxide (CO2) in solution form- carbonic acid (H2CO3), turning the bromothymol yellow-green. If carbon dioxide is removed from the solution, the solution will change to dark green and then to blue. Bromothymol blue will stain your hands and clothing. Practice safe laboratory procedures when performing this activity. 1. With a permanent marker, write your initials (or group symbol) on both of the capped vials. 2. Obtain an 8-cm sprig of Chara and place it in one of the vials provided. 3. Over a paper towel, fill both vials to overflowing with the yellow-green bromothymol blue solution and tightly. Take care not to spill on your hands and thoroughly clean up any spills. 4. Place both vials in sunlight or strong light source. 5. Wait one hour, and then answer the questions for Activity 1.

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter8: Forms Of Drugs And How They Act
Section: Chapter Questions
Problem 30RQ
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What does this reveal about the role of the carbon dioxide in photosynthesis? 

(the solution with chara is green so it is neutral while the btb solution is yellow so it is acidic )

Background and Procedure:
The chemical bromothymol blue is an indicator that appears blue in an alkaline (base) medium and yellow-
green in an acid medium. CO2 has been added to the bromothymol blue. Carbon dioxide (CO2) in solution forms
carbonic acid (H2CO3), turning the bromothymol yellow-green.
If carbon dioxide is removed from the solution, the solution will change to dark green and then to blue.
Bromothymol blue will stain your hands and clothing. Practice safe laboratory procedures when performing this
activity.
1. With a permanent marker, write your initials (or group symbol) on both of the capped vials.
2. Obtain an 8-cm sprig of Chara and place it in one of the vials provided.
3. Over a paper towel, fill both vials to overflowing with the yellow-green bromothymol blue solution and cap
tightly. Take care not to spill on your hands and thoroughly clean up any spills.
4. Place both vials in sunlight or strong light source.
5. Wait one hour, and then answer the questions for Activity 1.
Transcribed Image Text:Background and Procedure: The chemical bromothymol blue is an indicator that appears blue in an alkaline (base) medium and yellow- green in an acid medium. CO2 has been added to the bromothymol blue. Carbon dioxide (CO2) in solution forms carbonic acid (H2CO3), turning the bromothymol yellow-green. If carbon dioxide is removed from the solution, the solution will change to dark green and then to blue. Bromothymol blue will stain your hands and clothing. Practice safe laboratory procedures when performing this activity. 1. With a permanent marker, write your initials (or group symbol) on both of the capped vials. 2. Obtain an 8-cm sprig of Chara and place it in one of the vials provided. 3. Over a paper towel, fill both vials to overflowing with the yellow-green bromothymol blue solution and cap tightly. Take care not to spill on your hands and thoroughly clean up any spills. 4. Place both vials in sunlight or strong light source. 5. Wait one hour, and then answer the questions for Activity 1.
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