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- What is laser ablation and how can it he used to sample solids for ICPMS?A solution of KMnO4 with a concentration of 8.25 x 10-5 M yields an absorbance value of 0.1302 when measured in a 5.60 cm cuvette, set at a wavelength of 725 nm. Compute for the Molar Absorptivity Coefficient of the sample. Choices: a. 2.8182x10-8 c. 4.8182x10-8 b. 3.8182x10-8 d. 5.8182x10-8The absorbance values at 250nm of 5 standard solutions, and sample solution of a drug are given below: Conc. (ug/ml) A 250 nm10 0.16820. 0.32930 0.50840. 0.66050 0.846Sample. 0.661Calculate the concentration of the sample
- What percent transmittance range and what absorbance range are considered to be the optimum working ranges for spectrochemical measurements?What is the molar absorptivity of a 2.50 x 10-4 M solution if its absorbance at 550 nm is 0.500? The sample is placed in the standard 1-cm quartz cuvette. 2.00 x 10-3 M-1cm-1 5.00 x 10-4 M-1cm-1 2.00 x 103 M-1cm-1 1.25 x 10-4 M-1cm-1Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.
- Application of UV- VisibleSpectroscopyConcentration [M] Absorbance Solution 1 86.29 0.64 Solution 2 51.986 0.29 …. Fig 1: [graph of the calibration curve, make sure to show: function, R2, 0 intercept]Use least square regression to determine the best straight line and slope; compare the slope from 5 & 6. Concentration (M) Absorbance (no units) 0.10 0.09 0.20 0.14 0.30 0.19 0.40 0.27 0.50 0.30 0.60 0.34 0.70 0.37
- What absorbance corresponds to 1% transmittance? To 50% transmittance?The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample. 4.54 3.33 4.07 5.17 2.07Please create a caption for this table. Solution NaCl Conc. (%) Osmolality (mOsm) % transmittance Absorbance % hemolysis % crenation C distilled 0 0 0.001029 4.987584625 100 0.03354 1 0.177179111 54.61 0.001551 4.809388202 96.42720001 0.05837 2 0.297126222 91.58 0.01012 3.994819487 80.09527231 0.08444 3 0.442542222 136.4 3.849 1.414652089 28.3634704 0.134 4 0.590164444 181.9 64.8 0.188424994 3.777880643 0.2125 5 0.74752 230.4 95.64 0.019360433 0.388172513 0.3368 6 0.89644 276.3 99.56 0.001915112 0.038397585 0.5336 7 1.095648889 337.7 99.98 8.68676E-05 0.001741676 0.9834 8 1.336711111 412 100 0 0 2.1 9 1.755568889 541.1 100 0 0 7.9 10 2.674395556 824.3 100 0 0 57.83 11 4.490211111 1384 100 0 0 99.72
