You are observing the results from a standard plate count and you observe 53 CFUS. To make the plate count, you transferred 1 mL of a 10-4 dilution. How many CFU/mL are in the original sample? 1) 5.3 x 10-4 O 2) 5.3 x 103 O 3) 5.3 x 104 4) 5.3 x 105 O 5) 5.3 x 10-5
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- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap please
- Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.What is the likely result if you try to use the 100X objective without immersion oil?
- Why is there a need to use acids and boil the sample in maceration?Please explain: After making the 1:100 dilution, the laboratory scientist puts both the original serum sample and the labeled 1:100 dilution into the refrigerator. What is the appropriate temperature setting for the refrigerator? Choose the best letter answer and give a brief explanation. A. –10°C to – 15°C B. 1°C to 8°C C. 22°C to 24°C D. 37°CIf you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred 0.1 ml each time, what would be your dilution in tube 8?
- How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?Why are only few drops of CuSO4 solution added during the biuret test?