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What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which characteristic of the species used determines whether sterility was achieved when autoclaved?Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?
- What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?Why is the looping out method preferable for sputum specimen for acid fast smears?
- What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of pure cultures in thelaboratory?After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation?