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Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation? use the following Figures 1and 2 respectively to explain please

Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation? use the following Figures 1and 2 respectively to explain please

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation?

use the following Figures 1and 2 respectively to explain please

 

a IP: b IgG ARRB1 GST WB:p53 WB:ARRB1 GST-ARRB1 WB:GST WB:p53 GST- ARRB1 GST Nucleus ARRB1 Iso ARRB1 IP:MDM2 + p53 Merged WB:p53 WB:MDM2 IP:MDM2 Lysate Iso IP:MDM2 Lysate IP:MDM2 Lysate pcDNA Arrb1 WB:p53 Arrb1- Q394L WB:p53 WB:p53 WB:MDM2 WB:ARRB1 WT Arrb1-1-
Transcribed Image Text:a IP: b IgG ARRB1 GST WB:p53 WB:ARRB1 GST-ARRB1 WB:GST WB:p53 GST- ARRB1 GST Nucleus ARRB1 Iso ARRB1 IP:MDM2 + p53 Merged WB:p53 WB:MDM2 IP:MDM2 Lysate Iso IP:MDM2 Lysate IP:MDM2 Lysate pcDNA Arrb1 WB:p53 Arrb1- Q394L WB:p53 WB:p53 WB:MDM2 WB:ARRB1 WT Arrb1-1-
y-H2AX (arbitrary units) 2.5 2.0 1.5 1.0 0.5. 0 DNA damage Mouse thymus Iso LY294002- Nucleus P = 0.015 p53 protein levels (arbitrary units) + 1.2 1.0- p53 levels 0.8- 0.6- 0.4- WB:y-H2AX WB:histone 0.2- 0 f WB:p53 WB:histone WB:LDH Saline Iso P = 0.023 b Iso LMB 1 2 3 WT Nucleus Iso ICI Nucleus Iso ICI WB:p53 WB:B-tubulin + + + - + + H-89 + Ns Iso Iso Ns Iso Ns Iso WB:p53 WB:FOXO3a WB:p53 WB:histone WB:LDH 4 5 6 7 Arrb1-- Arrb2-/- WB:p-MDM2 (Ser 166) WB:MDM2 WB:p-MDM2 (Ser 166) WB:MDM2 Figure 1 Chronic catecholamine stimulation leads to p53 degradation and accumulation of DNA damage via ARRB1/AKT-mediated activation of MDM2. a, Isoproterenol infusion leads to accumulation of DNA damage and
Transcribed Image Text:y-H2AX (arbitrary units) 2.5 2.0 1.5 1.0 0.5. 0 DNA damage Mouse thymus Iso LY294002- Nucleus P = 0.015 p53 protein levels (arbitrary units) + 1.2 1.0- p53 levels 0.8- 0.6- 0.4- WB:y-H2AX WB:histone 0.2- 0 f WB:p53 WB:histone WB:LDH Saline Iso P = 0.023 b Iso LMB 1 2 3 WT Nucleus Iso ICI Nucleus Iso ICI WB:p53 WB:B-tubulin + + + - + + H-89 + Ns Iso Iso Ns Iso Ns Iso WB:p53 WB:FOXO3a WB:p53 WB:histone WB:LDH 4 5 6 7 Arrb1-- Arrb2-/- WB:p-MDM2 (Ser 166) WB:MDM2 WB:p-MDM2 (Ser 166) WB:MDM2 Figure 1 Chronic catecholamine stimulation leads to p53 degradation and accumulation of DNA damage via ARRB1/AKT-mediated activation of MDM2. a, Isoproterenol infusion leads to accumulation of DNA damage and
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