Agarose gel electrophoresis is a technique used to separate DNA fragments on an agarosematrix. The DNA is then viewed by staining or under UV light.(i)What is the direction of DNA migration in electric field on the agarose gel? · .(ii)Explain the principle for the migration of DNA molecules in an electric field.(iii)DNA loading dye is used to prepare DNA markers and samples for loading on theagarose gel. State the function of bromophenol blue in the loading dye.

i) DNA molecule is negatively charged molecule . So direction of DNA migration in electric field on…

Answered: Agarose gel electrophoresis is a…

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1CT

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Which of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?
What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?
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You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the binding in a binding buffer at pH 6.8. What portion (%) of the 3.0 µg of plasmid DNA would you expect to bind to the column in these conditions? What portion (%) of the bound plasmid DNA would you expect to elute using elution buffer at pH 8.5? Make the following assumptions:• Solid phase silanol groups can have a very wide range of pKas. For the purpose of this problem, assume the silica gel functional group (silanol) in the DNA purification column matrix has a pKa of 7.5.• If a DNA molecule and column matrix functional groups have the same charge state, then that DNA molecule will be repelled and will not bind to the column. If they do not have the same charge, binding occurs.
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