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If your DNA extraction were a concentration of 45ng DNA/ug, how would you prepare 500 ul of a 1 ng/ul solution of DNA in water?
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- How many kilobases of the DNA strand below will code for the protein product?How much buffer would you need to dilute a stock solution of DNA that is at a concentration of 150ug/ml to a final concentration of 8 ng/uL in 25 ml?You add 1 ul of EcoRI into 50 ul of your reaction of which contains 10 ug of DNA. How long would it take to completely digest the DNA?
- How many uL of DNA would I load into my gel if I am given a stock of 450ug/mL and I want to load 4ug?What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?IF A DOUBLE-STRAND DNA SAMPLE WAS COMPOSED OF 10 PERCENT THYMINE, WHAT WOULD BE THE PERCENTAGE OF GUANINE?
- Define which is the suitable pH to promote binding of DNA to the magnetic beads ?If DNA concentration is 2 grams, what would be the molar concentration of human DNA in a human cell?A reaction mixture contains DNA polymerase, the four dNTPs, and one of the DNA molecules whose structure is represented below. Which reaction mixtures generate PPi?
- If DNA concentration is 2 grams, what would be the molar concentration of human DNAin a human cell?Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.At 260 nm what component of DNA absorbs at this nm(hydrogen bond, phosphate backbone, nitrogenous base, deoxyribose).