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Please explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95% ethanol? Please include references.
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- What concentration of ethanol is considered optimum for disinfectant? Why not 100%?Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite some reasons.(Explain in at least 1 paragraph)Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:
- What are the different types of disinfectants? How is each one different or similar from the others? Explain and give examples too.Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?Compare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please explain phenol coefficient.
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?Agar is a solidifying agent used in media preparation. How and why does the agar concentration in semisolid media differ from conventional solid media?