Please help me with these questions, more than one answer may be correct for each: The above figures are showing the results BMAL1 gene knockout on the GFR and blood aldosterone in a mouse model over a 24-hour period (12h light, 12h dark).  ZT stands for Zeitgeber times, which is simply the number of hours since the beginning of the light period in the day.  GFR (dashed lines) was tested for a match to the modeled circadian cycle (solid line in top figure), and a p-value < 0.05 indicates a significant match (both control and knockouts were tested against the same modeled circadian cycle).  Significant differences between control (black) and knockout (red) mice blood aldosterone are marked with stars. 1) Genes/proteins that one could knockout that would likely have a similar effect include:   H ATPase   NA/K ATPase   PER   Anion exchanger   Cryptochrome 2) According to the figures above, knockout mice appear to:   have higher aldosterone production   lose circadian rhythmicity to GFR   live longer lives   have lower aldosterone production   have higher GFR

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 5.4P
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Please help me with these questions, more than one answer may be correct for each:

The above figures are showing the results BMAL1 gene knockout on the GFR and blood aldosterone in a mouse model over a 24-hour period (12h light, 12h dark).  ZT stands for Zeitgeber times, which is simply the number of hours since the beginning of the light period in the day.  GFR (dashed lines) was tested for a match to the modeled circadian cycle (solid line in top figure), and a p-value < 0.05 indicates a significant match (both control and knockouts were tested against the same modeled circadian cycle).  Significant differences between control (black) and knockout (red) mice blood aldosterone are marked with stars.

1) Genes/proteins that one could knockout that would likely have a similar effect include:

 

H ATPase

 

NA/K ATPase

 

PER

 

Anion exchanger

 

Cryptochrome

2) According to the figures above, knockout mice appear to:

 

have higher aldosterone production

 

lose circadian rhythmicity to GFR

 

live longer lives

 

have lower aldosterone production

 

have higher GFR

A
GFR (μl/min)
B
GFR (μl/min
C
Aldosterone (pg/ml)
380 Control
340
300
260
340
300
380BMAL1 knockout
260
450
300
0
150
0
4
600 ANOVA,
ANOVA genotype
p = 0.0095 I
Circadian fit;
p = 0.023
8 12 16 20 24
ZT (hours)
0
4 8
ZT (hours)
H
Circadian fit;
p = 0.818
12 16 20 24
T
4 8 12
HIO
第一重
ZT (hours)
*
16 20
24
Transcribed Image Text:A GFR (μl/min) B GFR (μl/min C Aldosterone (pg/ml) 380 Control 340 300 260 340 300 380BMAL1 knockout 260 450 300 0 150 0 4 600 ANOVA, ANOVA genotype p = 0.0095 I Circadian fit; p = 0.023 8 12 16 20 24 ZT (hours) 0 4 8 ZT (hours) H Circadian fit; p = 0.818 12 16 20 24 T 4 8 12 HIO 第一重 ZT (hours) * 16 20 24
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