Arrange the following compounds in order of elution from a column of silica gel: A) 1,3- dichlorocyclohexane; B) acetic acid; C) 3- hexanol; D) benzene.
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Give typed explanation
![Arrange the following compounds in order
of elution from a column of silica gel: A) 1,3-
dichlorocyclohexane; B) acetic acid; C) 3-
hexanol; D) benzene.
1.
2.
3.
4.
[Choose ]
[Choose ]
[Choose ]
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- A polymer P, consisting of two monodisperse fractions; fraction A with molecular weight of 1,000 g/mole and fraction B with a molecular weight of 100,000 g/mole. The batch contains an equal number of each fraction. Calculate the number average and the weight average molecular weight of the polymer. Describe the most important methods for preparing synthetic membranes. Which criterion is often used to choose the preparation method?Using a spectrophotometric technique, the phosphorus content in a mineral will be prolonged. The procedure used is described below: 8.35 g of sample are dissolved and treated so that all phosphorus is converted to the blue orthophosphate complex and made up to volume with distilled water end of 100 ml. The transmission of the resulting solution is 30.6%. If the orthophosphate standard solution of sodium, which is treated in the same way, and which has a final concentration of 2.467x10-4 M, gave a transmittance of 20.5%, calculate the phosphorus content in the driven sample in ppm Na3PO4.Despite their structural differences, separation of mixtures of organic compounds (such as the arenes benzoic acid, 4-t-butylphenol, and benzamide) often proves difficult. Silica gel chromatography, however, can be used with some success provided that an appropriate solvent is used to provide optimal separation. Consider, for example, the TLC plate pictured at right after developing and UV visualization where prior to elution, lanes A and B were spotted with 4-t-butylphenol and lanes B and C spotted with benzamide. Which of the following must be true? In the space provided, indicate the CORRECT answers (as 1, 2, and/or 3) then explain your reasoning for all three statements. Benzamide elutes faster than t-butylphenol. The Rf for t-butylphenol is less than that of benzamide. Benzamide is more polar than 4-t-butylphenol
- Calculate the values of vrms, vmp, and vmean for Cl2 (70.90 g mol-1) at 25 °C1.Why are the chlorophylls less mobile than the carotenes on the TLC plate? Give athorough explanation, including a sketch of how each type of molecule would interactwith the silica stationary phase material (you can use schematic structure formulas, ratherthan draw the whole molecules, which are quite large!). For instance, would one of thosetypes of molecules create more intermolecular force interactions than the other?Acetophenone is soluble in diethyl ether, isopropanol, and nhexane. Identify and justify the most suitable solvent for UV- Vis characterization?
- What is the structure and IUPAC name of atrazine? The herbicide simazine is more polar than atrazine. In reverse phase chromatography of a solution containing a mixture of the two substances, which would elute first? Explain why this is in terms of partition. The instrument used in this practical exercise has a UV spectrophotometer as a detector. Give two other types of chromatographic detectors that might be used.Why acetone has les retention time and toluene has more. discus about polarity of 3 samples and interaction with stationary phase1. The partition coefficient, k = (conc. in ligroin/ conc. in water), between ligroin and water for solute A is 6.5. (See Technique 10.1) A) What mass of A would be removed from a solution of 10 grams of A in 100 mL of water by a single extraction with 100 mL of ligroin? B) What mass of A would be removed by four successive extractions of 25-mL portions of ligroin? C) How much ligroin would be required to remove 98.5% of A in a single extraction?
- What are the general characteristics of Gas Chromatography, Thin Layer Chromatography, Ion Exchange, HPLC, SEC and Affinity Chromatography? What elutes first? Additionally can you explain what exactly is stationary phase and mobile phase?With the principles of GFC, which of the following statements is incorrect?a. For small molecules, their elution volume, Ve > VOb. For a very large molecule, its elution volume, Ve = Voc. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel.d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume.Pls explain whyAs a newly appointed research officer, Marissa has been assigned a task to separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high-performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.