With the principles of GFC, which of the following statements is incorrect? a. For small molecules, their elution volume, Ve > VO b. For a very large molecule, its elution volume, Ve = Vo c. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel. d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume.
With the principles of GFC, which of the following statements is incorrect? a. For small molecules, their elution volume, Ve > VO b. For a very large molecule, its elution volume, Ve = Vo c. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel. d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume.
Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter8: Thin-layer Chromatography: Analyzing Analgesics And Isolating Lycopene From Tomato Paste
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With the principles of GFC, which of the following statements is incorrect?
a. For small molecules, their elution volume, Ve > VO
b. For a very large molecule, its elution volume, Ve = Vo
c. Blue dextran is used to determine the void volume because it is too large
and cannot get into the beads and therefore excluded from the gel.
d. Low molecular weight molecules are freely accessible to the gel particles,
and elute at a volume equal to the internal pore volume.
Pls explain why
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