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Q: Microbiology
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Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
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Q: differential staining procedures
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Q: What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
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Please make an aseptic technique and pure culture technique flowchart
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- Need help list three N-removal Tertiary treatment approaches in order of decreasing cost of operation. State the microbiology (taxa and process) that is respinsible for the lowest cost process.I need help with microbiology What is the magnification of each of the following objectives? scanning: low power: high dry: oil immersion:Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:
- Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000Make an outline about culture-dependent and culture-independent methods in microbiology and explain it in detail. Include all the topics, subtopics, and examples involved in the two methods, respectively.The thermal death time in an autoclave at 121oC for a microbial culture was thirteen minutes. The thermal death time for another sample of the same culture in a hot-air oven at 121oC was twenty-one minutes. Use your knowledge of what thermal death time measures to identify which method of heating was more effective against this microbial culture. The hot-air oven treatment was more effective. The autoclave treatment was more effective. Both thermal death times were equally effective. Not enough information is provided to determine effectiveness.
- A culture medium that does not grow gram-positive organisms would be a differential plate enrichment plate selective plate streak plate pour plateCreate a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact with the speciman none of these heating the inoculation loop until red heat fixation of the speciman flaming the lip of a test tube cultureTechnique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!