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Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture can be stored.
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- Distinguish batch culture and continuous cultureWhat is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?
- Why is dilution a necessary part of pure culture preparation?Give atleast two other examples of samples that are best prepared using Smear Preparation Technique.What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- Define a pure culture.Write an introduction related to culture media and isolation of pure culture technique (streak plate and spread plate method)In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?