Describe (at the cellular level) how detergents like roccal and SDS (from solution 2 in the plasmid extraction) sterilize surfaces. (Answer for each detergent.)

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: The column chromatography procedure is used to separate a single chemical compound from a mixture.…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…

A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…

Q: The colonies growing on the surface of solid media represent: Select one: a. A group of different…

A: The CFU/ml are often calculated using the formula:cfu/ml = (no. of colonies x dilution factor) /…

Q: Bacterial techniques, plasmid isolation, digestion and gel electrophoresis Answer all with…

A: 1) Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria.…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…

A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…

Q: Why is it important to use a sterilized loop between streaks when preparinga streak­plate?

A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: Practical techniques used to identify unknown microorganisms’ You should include the types of tests…

A: Microbiology is the study of microorganisms, identification, and classification of a particular…

Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…

A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…

Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in…

A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: Which of the following agents if introduced into a growing culture of bacteria would halt growth but…

A: INTRODUCTION Bacteria, also called germs, are microscopic organisms not visible with the naked eye.…

Q: Given the following common laboratory materials, which of the three methods of heat sterilization do…

A: Sterilization is the used to kill the microorganisms or other pathogens from a substance or…

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…

A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…

Q: Which among the following demonstrates the difference between Native Polyacrylamide Gel…

A: PAGE or polyacrylamide gel electrophoresis is a separation technique under the influence of…

Q: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing…

A: a. Preparing/determining the concentration of the agarose gel- The more the concentration of the…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…

A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…

Q: Explain the function of stacking gel, separating gel, and Coomassie blue stain in the…

A: Answer : Stacking Gel : The purpose of the stacking gel packing is to combine all the samples of the…

Q: When labeling culture tubes or plates why do we use a pencil instead of a pen?  Are there other…

A: Appropriately labeling specimens for research labs is of critical importance.

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: During the fall 2001 bioterrorist attack in which anthrax endospores were sent through the mail, one…

A: Introduction: Spores are the extremely resistant resting-state that are made at the time of…

Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…

A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…

Q: Staining bacterial capsule can not be accomplished by?

A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: Uses of tannins consist of: Select one: a. leather tanning and ink manufacturing b. Astringent,…

A: Tannins: Tannin is also called as tannic acid that acts as a deterrents and is used for various…

Q: Imagine that you are asked to design a surface (BUT ONLY SURFACE) pasteurization process of eggs.…

A: The process by which the pathogenic microorganisms are destroyed from certain food items is called…

Q: What is the best streaking technique in a culture media? Explain

A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…

Q: What is the use of a “ladder” on a gel electrophoresis?

A: When there is a mixture of nucleic acids and proteins, they can be separated according to their…

Q: ative gel electrophoresis

A: Separation techniques are critical for identifying and isolating biomolecules for further biological…

Q: You treat cells briefly with radioactively labeled cysteine and then transfer them to a medium with…

A: Cysteine is a nonessential amino acid. It is one of the amino acid that contains sulfur which allows…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

Q: The DNA fragments separated on an agarose gel can be visualised after staining with what?

A: Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis…

Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…

A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…

Q: Briefly describe the principle and the procedure of organic (Phenol-Chloroform) DNA extraction…

A: DNA Extraction: It is the process of isolating DNA from a cell. There are several techniques…

Q: Give the three disadvantages of using irradiation.

A: Irradiation is a comparatively newer technology of food preservation in which food item is…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: Which of the following items is the least suitable for sterilization by autoclave? a. Water for…

A: An autoclave is a machine that is used to perform industrial and scientific processes that require…

Q: You have been directed to take a sample from a growth-free portionof the zone of inhibition in the…

A: Penicillin: It is a group of antibiotics. From Penicillium molds, penicillin derived. Different…

Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…

A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…

Q: Congo red is a red acidic stain and methylene blue is a l a bacterial smear with a mixture of eosin…

A: Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective…