SIZE ESTIAMTION- Since we know the diameter of the field of view, we can estimate the size of a specimen by determining the proportion of the field of view the object (cell) takes up. Based on the diameters in um above, estimate the size of the following object as if looking through the 1. low power 2. high power 3. oil
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- Question:- We are using in class 4.5 mm and 40X. a) How big is a specimen that takes up 1/2 the field view at 90X?True or false? each objective of a phase contrast microscope contains a phase plate. most cultured mammalian cells grow best at 37C. the oculars on the compound light microscope are typically 10X.Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.
- Question:- calculate the distance between two ocular micrometer division markers in micrometers (μm) when viewed at 100x total magnification, then at 400x.Do the bottom diagram Find all th calculations Micrograph width is 0.2mm at 400x Draw a micrograph diagram and calculationsYour slidebjs in focus on the microscope, but you do not see the specimen. 1. What are the possible problems 2. What are the possible solutions
- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?Please answer fast What are some similarities and differences in Bradford assay and Nanodrop (to obtain direct absorbance measurements). What are the benefits and setbacks of them. How is 260/280 absorbance ratio significant?disadvantage of Rees and Ecker method.
- Copy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48 Gel electrophoresis Questions 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?True or false ? images will appear brighter when light waves are in phase. the condenser portion of the microacope focuses light on the speciment.FRAP can be used to analyze living cells.a) Trueb) False