Put the following steps for running a gel in order. first Remove comb and put gel int v Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel second third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

Put the following steps for running a gel in order. first Remove comb and put gel int v Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel second third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them
Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
What are the things you need to consider when doing the following: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing the stain- d. Placing the electrodes on the power source- Briefly describe the function/s of each of the following: e. Gel f. Comb g. Stain h. Running buffer j. Molecular weight ladder/DNA size standard k. Micropipette l. Tracking dye m. Anode (red) n. Cathode (black) o. Microwave oven
Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > Glycine
In SDS PAGE the resolving gel : is 4%(w/v) acrylamide,PH )6.8 is 6-20% (w/v) acrylamide is 4%(w/v) acrylamide
Your slidebjs in focus on the microscope, but you do not see the specimen. 1. What are the possible problems 2. What are the possible solutions
Place the following steps for setting up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel running apparatus . I. Add Sybr-safe to the molten agarose. J. Connect the leads to both the gel running apparatus and the power pack .
State the functions for each of these components used in the gel filtration chromatography using a column experiment. Bromophenol blue A mixture of albumin and bromophenol blue Spectrophotometer
Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!
How do we ensure that gel electrophoresis is functional? a Running buffer, containing electrolytes, allows for electrical current. b The apparatus must be either vertical or upright. c The matrix of the gel must be set to the appropriate percentage. d All proteins need to be denatured and given a unique charge.
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel? 2. What is a gradient gel? Why are we using them? 3. Compare and contrast SDS-PAGE and native PAGE. Why would we want to do each of these techniques?