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Help Answering
Why is it important for all plankton monitors to use the sample protocol for both sample collection and identification?
What are the ways you can control the amount of light passing through your specimen?
What is resolution and how is it improved?
With which objective is it permissible to use the course adjustment knob?
Many of the plankton images were taken using the 40x objective on a compound microscope. What is the total magnification?
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- Questions: Which samples can have their own (innate) fluorescence? Part of the spectrophotometer that transmits the desired wavelength: a.monochrome b.Selector c.Detector d.prismTrue or false? each objective of a phase contrast microscope contains a phase plate. most cultured mammalian cells grow best at 37C. the oculars on the compound light microscope are typically 10X.True or false? light microscopy has a limit of resolution of 200nm. phase contrast microscopy can be used to observ thick (more than 2 cells)speciments. mammalian cells are grown in a humidified incubator at 30C and 5% CO2.
- Please answer fast What chemical is the mordant in the Gram staining procedure. Explain how it functions during this staining.Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL QUESTION Using the image: 5. Identify whether the organism is a green alga, a diatom, a dinoflagellate, Eubacterium, or a Cyanobacterium. Explain the characteristic(s) that led you to that identification. 6. a. What type of microscopy (brightfield, darkfield, or phase contrast) was to obtain the image? Explain how you can tell. b. Name a different type of microscopy would be also appropriate to use to visualize the organism in the image. How would the image change if you used that type of microscopy instead? 7. The image was taken using a microscope that has 10X, 40X, and 100X objectives. What is a possible magnification for the ocular lens on this microscope? Explain your response or show calculations.
- Instruction: FILL IN THE BLANKS 1. For initial focusing of the specimen the ___________ (objective) is aligned with the body tube and the central opening in the __________. 2. To view the specimen on the stage, secure the slide with the __________. Make sure the slide is in line with the ____________ and the stage opening. 3. Adjust the ______________ to regulate the amount of light required. Adjust the _____________ properly so that optimum light is reflected in the microscope. 4. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray. 5. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed. 6. In shifting to higher magnification, simply…Which of the following statements regarding electron microscopy is FALSE? Question options: live organisms cannot be viewed with an electron microscope all of these statements are TRUE scanning electron microscopes focus electrons across the surface of a sample electrons pass through a sample viewed using transmission electron microscopy electron microscopes use magnetic fields in place of glass lenses electron microsopes have a better resolving power than light microscopesCopy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48 Gel electrophoresis Questions 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
- Please help using the image i attached below. There are 3 parts to this question. Describe how this is related to light scattering. Note the field is always dark in this type of microscope (and hence, called dark-field). Why is this? Explain how this type of microscope can be used to detect (but not resolve) flagella.True or false ? images will appear brighter when light waves are in phase. the condenser portion of the microacope focuses light on the speciment.A lab revolving around compound microscopes was conducted. Please answer the following questions (questions 1, 2, and 3). If a specimen (placed on a slide) has little or no color, what level of light intensity should you use; should the intenisty of the light be high or low (choose)? Which objective should be in position before you start to focus the microscope? Which focus knob should you use first with this objective?