Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract. A. THIN LAYER CHROMATOGRAPHYEarth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubiatinctorum, commonly known as rose madder. Earth spotted the root extract on twohydrophobic silica gel plates. He then placed each plate in separate developing chambers,one containing solvent system WHITE and the other housing solvent system MIX. Earthallowed the chromatograms to develop for around five minutes. Afterwards, he removedthe plates from the containers and placed them inside an iodine chamber. Thechromatograms resulting from the TLC experiment after visualization are illustrated asfollows. The distances travelled by each spot and by the solvents were measured andrecorded in the displayed table.D75.0 mm71.5 mmDISTANCESOLVENTSPOTSTRAVELLED FROMSYSTEMsolventTHE ORIGIN, mmfrontA13.0В32.5WHITE41.0D66.5A10.5B27.5MIXA- origin,A39.0D53.510.5 mm8 mmWHITEMIXO mm-O mm

A question based on purification, which is to be accomplished.

Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.

Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter4: Least-squares And Calibration Methods

Section: Chapter Questions

Problem 3P

See similar textbooks

Similar questions

Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubiatinctorum, commonly known as rose madder. Earth spotted the root extract on twohydrophobic silica gel plates. He then placed each plate in separate developing chambers,one containing solvent system WHITE and the other housing solvent system MIX. Earthallowed the chromatograms to develop for around five minutes. Afterwards, he removedthe plates from the containers and placed them inside an iodine chamber. Thechromatograms resulting from the TLC experiment after visualization are illustrated asfollows. The distances travelled by each spot and by the solvents were measured andrecorded in the displayed table.
separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.
As a newly appointed research officer, Marissa has been assigned a task to separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high-performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.
A brewed tea brand sample was subjected to solvent extraction to extract caffeine. The cup of tea measuring 200 mL was extracted with petroleum ether. With KD = 20.0 (caffeine in pet ether-water), the % caffeine (194.10) extracted after 3 extractions with a total of 75.0 mL pet ether used is: A. 97.7% B. 28.6% C. 71.4% D. 2.33%
Extraction of the aqueous salicylic acid solutions with 10% ethyl acetate/hexane (density ~ 0.7 g/ml) will give two layers in the separatory funnel. Will the aqueous layer be the upper layer or the lower layer? If dichloromethane (density ~ 1.3 g/ml) were substituted for the 10% ethyl acetate/hexane solution, which layer would be the aqueous layer?
For thin layer chromatography, why should a piece of filter paper that is moistened with the developing solvent be placed inside the developing chamber before the plate is developed? Group of answer choices to absorb excess solvent from the TLC chromatogram plate to absorb any impurities in the eluent to signal completion of the experiment to ensure that the atmosphere of the chamber is saturated with eluent vapor
What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for Analysis of MSG from Beef Broth Cubes, including those of other ions like NaCl? What stationary phase and mobile phase (together with elution technique), and other chromatographic conditions needed?
A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 3.40×102 and the area of peak B is determined to be 398 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.49 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 437 for peak A and 415 for peak B. What is the concentration of methyl benzoate in the plant stream?
Why ebuilliometer is having a great effect on measuring ethanol Yield than gas chromatography
So we lately had a paper chromatography activity and we used a plant with only one pigment (which is red). It was a blot-dry technique using water and ethyl alcohol. Describe the chromatogram used and the process of indicating the point of origin and the solvent front. Describe how the sample was applied using the "Blot-dry" technique. Describe the solvent system or the solvent used in developing inside the chamber.
Gravimetric factor:A 1.0125 g portion of the sample was oven-dried leaving 0.9385 g of dry matter. In a similar experiment a 2.0163 g portion of the sample was oven-dried and extracted with a non-polar solvent. the solvent was evaporated leaving a 0.8444 g residue. what is the amount of crude fat present in the feed? (in %w/w, dry matter basis)
A Tlc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography thatwas done. The Tlc plate was developed in 20% ethyl acetate/80% hexanes and is shown below.