Below is a picture of ELISA results. Why is the color darker in some wells and lighter in others? Assume that all of these wells are test wells, not controls.
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- Why are two negative controls included in an ELISA testWhat other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA test? Name and very briefly describe one other test. Give the reason for your choice.Why is important to note the Kit / Lot number and expiration date for each kit or tests done from each ELISA kit?
- What other test can be used to confirm the results obtained in this ELISA test? Name and very briefly describe one other test. Give the reason for your choice.in an indirect elisa procedure what enzyme is used?In a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) Antigens
- What would you use for a positive control in any ELISA test? What would you use for a negative control?All of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibodyWhat are the four steps of an Elisa protocol
- What is the role of horseradish peroxidase conjugate in the ELISA experiment? Antigen Primary antibody Second antibody SubstrateWhich of the following best describes a positive reaction in an ELISA? A color change takes place. All of the molecules in the ELISA coagulate. There is a pH change. If the color of the ELISA does NOT change, the result is positive.How does a positive reaction in an ELISA test appear? How manywells are positive in figure ?