Bio If you plated 100 uL of bacteria from a 10-5 dilution and count 73 colonies after incubating for 24hrs, what is the CFU/mL of the original (undiluted) culture? Question 3 options: 7.3
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Bio
If you plated 100 uL of bacteria from a 10-5 dilution and count 73 colonies after incubating for 24hrs, what is the CFU/mL of the original (undiluted) culture?
Question 3 options:
7.3
7.3 x 10^7
7.3 x 10^-6
0.73
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Solved in 3 steps
- Computational True or False. UHT-pasteurized milk sample X which was spread-plated in quadruplicates yielded the following bacterial colony counts: 23, 20, 35 and 41 CFU/plate at a dilution factor of 100. Therefore, the milk sample will pass laboratory standards if the quality control laboratory sets the total microbial limit as: “not to exceed more than 30,000 CFU/mL of milk sample”.COMPUTATIONAL TRUE OR FALSE Milk sample A contained an average of 53 cells per FOV at a dilution factor of 7 whereas Milk sample B contained an average of 99 cells per FOV at a dilution factor of 5. Therefore, milk sample A has less microbial contamination. (Note: 0.01mL of each sample was placed on a clean glass slide and both samples were examined under OIO with an area of 1.77x10-4 cm2.)Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to 4.6x108 CFU/mL in 3 hours. Be sure to use units in your answer.
- If a broth culture of bacteria, initially containing 37,000 bacteria/ml, were diluted to a 1:1,000 dilution, how many bacteria/ml of the diluted broth would be present on average? Show your solutionsYou did a serial dilution experiment to determine the CFU/table of the Webbers' probiotics. Given the ingredient list shown, how many colonies would you expect to see on a plate if you plated 0.1 mL from the 10-6 dilution tube, and incubated aerobically at 37 ⁰C for several days?You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?
- Multiple Matching. For each type of medium, select all descriptions that fit. For media that fit more than one description, briefly explain why this is the case. _______mannitol salt agar a. selective medium _______chocolate agar b. differential medium _______MacConkey agar c. chemically defined (synthetic) _______nutrient broth medium _______brain-heart infusion broth d. enriched medium _______Sabouraud’s agar e. general-purpose medium ________triple-sugar iron agar f. complex medium ________SIM medium g. transport mediumCompare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
- Voges Proskauer test Does color of the medium change to red after both reagents were added and allowed to sit for 60 minutes? If yes, after about how many minutes before you can see red color?you did a serial dilution experiment to determine the CFU/table of the Equate Children's probiotics. Given the ingredient list shown, how many colonies would you expect to see on a plate if you plated 0.1 mL from the 10-7 dilution tube, and incubated aerobically at 37 ⁰C for several days?Compute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values below. Show your solution. Media First Sampling First Sampling First Sampling Second Sampling Second Sampling Second Sampling 10-2 10-3 10-4 10-2 10-3 10-4 VRBA TNTC, TNTC 243, 232 24,22 23, 25 0, 0 0, 0 MRS 32, 40 0, 0 0, 0 TNTC, TNTC TNTC, TNTC 102, 120