Answered: Name some techniques/approaches (name,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Related questions

Question

Name some techniques/approaches (name, describe the major steps, how to analyze/interpret results) that can be used to satisfy the following requirements:

1. Show that substance is pure protein

2. Only one protein was extracted

Expert Solution

Step 1

Proteins:

Proteins are polymers that contain hundreds or even thousands of amino acids that are linked by peptide bonds.
We need to purify proteins to study the structure and function of the protein of interest.
We get proteins from the whole organism, organs or tissues, tissues culture cells, and proteins from expression systems.
Three important steps are necessary for protein purification: solubilization of protein, stabilization, and isolation in its pure form.
Some of the applications of purified proteins are their use in diagnostic purposes, such as cellular markers, vaccines, and recombinant DNA technology to locate genes of interest.