A stock culture of S. aureus was serially diluted up to 10'you spread plate 0.1ml in the two nutrient agar Petri dishes and obtained 20 and 40 colonies, respectively. After 24 hours, the stock culture of S. aureus was serially diluted again up to 10 and 0.Iml was spread plated to the two Petri dishes and obtained 100 and 150 colonies, respectively a. That is the cfu/ml in the initial population of S. aureus b. What is the cfu/ml after 24 hours c. What is the generation time?
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- A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a sample was taken directly from the milk and plated onto nutrient agar and the following day after incubation 98 colonies were counted. What is the CFU/ml of the S. typhimurium in this sample?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells were counted in 25 small squares (each of volume 2.5 x 10-7 cm3). Viable counts were carried out on the same culture using both the pour plate method (incorporating 1 mL samples of a range of dilutions into plate count agar plates) and the Miles & Misra spread plate method, where 0.02 mL samples were spread on sectors of a plate count agar plate. For the pour plate count the average of triplicate samples was 178 colonies per plate of the 10-4dilution. For the Miles and Misra count the average of triplicate samples was 21.3 colonies per sector of the 10-4 dilution. Calculate the total count and the viable pour plate and spread plate counts and suggest possible reasons for the difference between the three different counts.
- volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)Below is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asapA culture of E. coli is diluted as follows:(1) 65mL are added to 435mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-3 dilution using 1000 uL from the original culture?
- In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
- You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the different types of bacteria present in the mixed culture without using an agar plate 1b. How will you make a pure culture of these bacteria on a slant nutrient agar 1c. How will you identify these bacteria from the pure culture without using an agar plateSuppose you have a pure culture of Serratia marcescens. You subject this culture to both streak plate technique and viable counting technique. State the one common goal or end result of these two techniques.Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by 4 successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample? •Assume: 1 g = 1 mL •Spread plate uses 0.1 mL