Biotechnology is a constantly developing field with many exciting applications. It focuses on the use of living organisms and their biological processes to make useful products and address practical problems. This includes advancements in clinical diagnostics, epidemiological tracking, forensics, industry, and many other areas. In this simulation, you will have the opportunity to perform molecular techniques commonly used in the field of biotechnology to create medically important compounds such as insulin and human growth hormone.Bacteria can obtain naked DNA from their environment in a process called transformation. This process occurs naturally but can also be used in the genetic engineering field to alter the genetic makeup of bacteria. In this simulation, you transform bacteria with a jellyfish gene whose product is green fluorescent protein. The transformed bacteria now have the genetic information to produce GFP (green fluorescent protein), causing them to glow green under fluorescent light. a) explain in your own words how to prepare sample for transformation b) explain in your own words how to  Perform heat shock and bacterial recovery c) explain in your own words how to incoulate plates with positive pGLO and negative pGLO

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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Biotechnology is a constantly developing field with many exciting applications. It focuses on the use of living organisms and their biological processes to make useful products and address practical problems. This includes advancements in clinical diagnostics, epidemiological tracking, forensics, industry, and many other areas. In this simulation, you will have the opportunity to perform molecular techniques commonly used in the field of biotechnology to create medically important compounds such as insulin and human growth hormone.Bacteria can obtain naked DNA from their environment in a process called transformation. This process occurs naturally but can also be used in the genetic engineering field to alter the genetic makeup of bacteria. In this simulation, you transform bacteria with a jellyfish gene whose product is green fluorescent protein. The transformed bacteria now have the genetic information to produce GFP (green fluorescent protein), causing them to glow green under fluorescent light.

a) explain in your own words how to prepare sample for transformation

b) explain in your own words how to  Perform heat shock and bacterial recovery

c) explain in your own words how to incoulate plates with positive pGLO and negative pGLO

 

 

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