Q: What are the applications of transformation?
A: In genetics and molecular biology, transformation is genetic alteration of a cell. It results from…
Q: Explain the methodology, rationale, and challenges in the control of Triatomine vectors.
A: Triatomine: Triatominae bugs are the vectors of Chagas disease. These belong to the Reduviidae…
Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?
A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…
Q: Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays,…
A: Biuret, lowry, and bicinchoninic acid-based assays are used to quantify the protein concentration in…
Q: Explain Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels.
A: Peptide mapping is an identity test for proteins, those obtained by rDNA technology. It involves the…
Q: What is the difference between ORF and CDS?
A: ORF (open reading frame) is the portion of a reading frame that has ability to be translated. CDS…
Q: Explain briefly the hershey-chase experiment?
A: The confirmation that DNA is the genetic material was given by the Hershey-Chase experiment. The…
Q: what is the biochemical basis for affinity purification of a recombinant protein using a 6X his tag…
A: The DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors…
Q: Briefly describe the importance of two dimensional electrophoresis in protein separation?
A: Two-dimensional gel electrophoresis is the technique that is used in the separation of the proteins…
Q: What is transformation efficiency and how is it calculated?
A: Transformants are the cells that are able to incorporate any modified foreign or artificial DNA.…
Q: What types of homogenization techniques are available for solubilizing a protein?
A: In the process of slating out, the solubility of the protein reduces due to the highly ionic salt…
Q: Using sickle-cell anemia as a basis, describe what is meant by a genetic or inherited molecular…
A: A disease is an abnormal condition of the body or body that does not work properly and causes a…
Q: What would happen during an amino acid sequencing experiment using the Edman degradation if you…
A: The sequence of amino acids can be determined by using amino acids which are present in peptide and…
Q: a BLASTP
A: Introduction The Basic Local Alignment Search Tool (BLAST) observes districts of nearby similitude…
Q: In microbial genetics, what is referred to as Griffith effect?
A: Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics…
Q: Describe the two general types of protein microarrays. What aretheir possible applications?
A: A protein microarray is also known as the protein chip, is the high throughput method that is used…
Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.
A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…
Q: Describe in detail the preliminary purification method which you will use to partially purify a very…
A: Proteins are polymers made by combining amino acids as the monomeric units. Amino acids are combined…
Q: What are the two advantages of using sequence analysis of ribosomal components in determining the…
A: Ribosome is an essential component of cellular machinery that is present across all life forms. The…
Q: Briefly explain in situ hybridization, giving some applications of this technique.
A: Introduction Molecular biology deals with various techniques for study of structure and functioning…
Q: What is the formula for transformation efficiency?
A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…
Q: Outline the proofreading processes in amino acidactivation.
A: Genes are the unit of heredity. The genetic information present in DNA is copied into a molecule of…
Q: Explain Needleman-Wunsch and Smith- Waterman programming algorithms. What is a substitution matrix?…
A: The process in which two or more sequences (DNA, RNA and proteins) are compared in order to find…
Q: In biochemical and structural terms, how does the cellular form of the prion protein differ from the…
A: There are different macromolecules present in the living organism. Of which proteins are one of the…
Q: why are 2D NMR spectra symmetrical
A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…
Q: What are two ways to denature a protein and why do these methods work?
A: Given: Two ways to denature a protein.
Q: Please briefly describe the content of Watson-Crick Proposal.
A: James Watson and francis crick were pioneers in genetics as they discovered the three dimensional…
Q: Dichotomous key?
A: The dichotomous key is an important scientific tool, used to identify different living things, based…
Q: What is Hybridization/Annealing ?
A: Annealing which is also known as Hybridization is defined as the spontaneous pairing occur of…
Q: CPT code assignment 44970
A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…
Q: Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant…
A: Introduction: Liposomes are a small-sized vesicle sharing structural and compositional similarity…
Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?
A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…
Q: Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as…
A: One of the techniques for figuring out the sequence of DNA or RNA is called next-generation…
Q: What are some advantages of sequencing peptides by mass spectrometry rather than by Edman…
A: Edman degradation: It is a three-stage reaction that tags and removes the N-terminal residue of a…
Q: Consider the following wild-type and mutant sequences:Wild-type ....CTTGCAAGCGAATC....Mutant…
A: Introduction A mutation occurs when the sequence of DNA changes. Mutations can occur as a result of…
Q: Intrinsically disordered regions of proteins can be identified using bioinformatic methods to search…
A: Despite the classical structure-function paradigm (which is often visualized because the…
Q: What method could be used to make an immobilised library of 6 different peptides, all differing by…
A: INTRODUCTION Peptide-based molecular probes identified by bacteriophage (phage) display technology…
Q: Why does they errors occur?
A: Safety errors can be caused by a myriad of reasons in ambulatory healthcare. We need to constantly…
Q: Contrast the contributions made to an understanding of transformation by Griffith and by Avery and…
A: Microorganisms or microbes have various kinds of interaction with other living things. They exhibit…
Q: In reversible terminator sequencing, how would the sequencing process be affected if the…
A: The basic between sanger's sequencing and reversible terminator sequencing is that Sangar's based on…
Q: Why is the induced-fit model a more likely model than the lock-and-key model?
A: In the chemical reaction, the enzymes help to catalyze the reaction by increasing the rate of…
Q: What factors could explain a transformation efficiency?
A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…
Q: Can you describe the ensemble allostery model and explain how it is used to explain allostery in…
A: In allosteric regulation binding of a ligand to one of the subunit enhances or decreases the binding…
Q: what are the advantages and disadvantages of using recombinant protein and affinity chromatography…
A: Recombinant proteins are produced by recombinant genes. Affinity chromatography allows particle…
Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification…
A: Protein purification is a process of isolation of specific protein from the complex protein mixture…
Q: Describe two different types of protein microarrays, and discuss their uses.
A: Genomics and proteomics are relatively new branches of genetics. Genomics is the study of all the…
Briefly discuss the applications of k-mer analysis.
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Solved in 2 steps
- Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as Semiconductor (Ion Torrent) sequencing.Discuss the disadvantages and advantages of both 2D-DIGE and 2D-PAGE.Explain briefly why the derivation of protein substitution matrices introduces a form of circular reasoning linked to the 'alignment paradox'
- List and Describe the algorithm of BLOSUM substitution matrix computation.Describe the common strategy (steps) for protein sequencing, starting with a biological sample containing many cell and biochemical substances. How prevalent are disulfide links in proteins? Why do the disulfide links need to be broken prior to sequencing? How can they be chemically broken?For the CPT code assignment 44970, which of the following coding references can be accessed from the coding summary screen? Select all that apply.