Q: Name one common methods in DNA/RNA extraction. What is the Difference between DNA Purification and…
A: The process of “DNA extraction” is way of isolating and purifying DNA. There are many ways to…
Q: Calculate the melting temperature of this primer and estimate the annealing temperature of this…
A: Introduction PCR (polymerase Chain Reaction) is a molecular technique by which multiple copies of…
Q: what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?
A:
Q: What are the applications of transformation?
A: In genetics and molecular biology, transformation is genetic alteration of a cell. It results from…
Q: what is a replicase enzyme (protein)?
A: Replicase is an enzyme that has the capability to replicate nucleic acids. Enzymes are usually…
Q: What is a poor transformation efficiency?
A: Transformation is a natural phenomenon mostly observe in many bacteria. It is dependent on the…
Q: What is the meaning of DNA clone?
A: Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains that coil around…
Q: What is the point of hydrolyzing RNA before doing biochemical tests?
A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.
Q: What is the difference between ORF and CDS?
A: ORF (open reading frame) is the portion of a reading frame that has ability to be translated. CDS…
Q: What Is a DNA Library?
A: For constructing DNA libraries, the DNA is processed using restriction enzymes. The components of…
Q: In preparation for transformation, why do you think we used calcium chloride and magnesium chloride…
A: We often use calcium chloride and magnesium chloride to make cell competent. As DNA is hydrophilic…
Q: What is the function of conjugation reactions in the biotransformation process?
A: Biotransformation refers to reactions that modify a specific chemical compound (both endogenous and…
Q: What is the difference between positive and negative supercoiling?
A: The DNA (Deoxyribonucleic acid) is the genetic material that is inherited by offspring over…
Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…
A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…
Q: What do you mean by restriction fragments?
A: Recombinant DNA technology is the process of combining two or more DNA fragments from a different…
Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…
A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…
Q: What is the hybridization and synthesis process and why it is needed?
A: In the cell, there are several processes, which are required for survival and biochemical functions.…
Q: What does SDS–PAGE stand for? What is the benefit of doing SDS– PAGE?
A: Electrophoresis is the migration of charged particles under the influence of an electric current.…
Q: What is the formula for transformation efficiency?
A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…
Q: Briefly discuss the applications of k-mer analysis.
A: k-mer analysis is used in the field of bioinformatics, computational genomics, and sequence…
Q: What different between the generated amplification product and the primer sequences in the CDS…
A: An increase in the number of copies of a gene in a genome is referred to as gene amplification. In…
Q: What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each…
A: The DNA (deoxyribonucleic acid) is the hereditary nucleic acid sequence that contains the genetic…
Q: What is transformation? How did Avery and his colleagues demonstrate that the transforming principle…
A: In some species of bacteria transformation is naturally occurring process. Transformation is used as…
Q: Which of the following is defined as transformation?
A: Which of the following is defined as transformation? Answer. Option (C) . Free-floating DNA is…
Q: why are 2D NMR spectra symmetrical
A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…
Q: What are the three main steps in procedures of transformation?
A: Bacterial transformation is a horizontal gene transfer wherein certain bacteria called competent…
Q: Dichotomous key?
A: The dichotomous key is an important scientific tool, used to identify different living things, based…
Q: In a protocol for DNA sample preparation for agarose gel electrophoresis, what volume of 4X loading…
A: Electrophoresis It is a biochemical technique to separate charged molecules under the influence of…
Q: What increases transformation efficiency?
A: Transformation efficiency refers to the efficiency by which a cell can easily take up a…
Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…
A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…
Q: What are biochemical characterisation of transforming principle?
A: Frederick Griffith conduct a series of experiments with bacteria streptococus pneumoniae (or…
Q: What is the purpose of a standard curve for a colorimetric enzyme assay?
A: Enzyme assays are mostly used to determine the vitamin status in the body. The activity of…
Q: What is Kozak sequence ? who invented this ?
A: Introduction The process of transcribing the mRNA into protein is called Translation. There are 3…
Q: What does A230 indicate, in assessing extracted DNA quality?
A: Option C Extraction contaminants
Q: When is the transformation factor used?
A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…
Q: Who revealed biochemical nature of the transforming principle?how was it done?
A: The genetic material refers to the substance present in mitochondria, cytoplasm, and nucleus. It…
Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?
A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…
Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?
A: Introduction: RNA is a type of nucleic acids that is a genetic material in some viruses.
Q: Contrast the contributions made to an understanding of transformation by Griffith and by Avery and…
A: Microorganisms or microbes have various kinds of interaction with other living things. They exhibit…
Q: What does it mean to hydrolyze RNA before doing biochemical tests?
A: The nucleic ribose acid is RNA. It is a nucleic acid type. There are three kinds of RNA: reran,…
Q: What factors could explain a transformation efficiency?
A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…
Q: What is reductive dechlorination and why is it environmentallyrelevant?
A: Chlorination refers to the process of the addition of chlorine into the water for the disinfecting…
Q: What is the primary disadvantage of Sanger sequencing?
A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…
Q: What does A280 indicate, in assessing extracted DNA quality?
A: The correct answer is aromatic amino acids.
Q: what is isosterism and bioisosterism? how does this concept help in biotransformation of different…
A: The term Pharmacokinetics is used to refers to the measurement of the fate of a pharmacological…
Q: What is the functional significance of sticky ends in a cloningexperiment?What type of bonding makes…
A: Answer- Sticky ends are generated after restriction endonuclease is applied on a DNA on the…
Q: What is natural transformation in biology?
A: In biology transformation is the process of genetic alteration of a cell. In this process an…
Q: What is deadenylation-independent decay? What is the role of deadenylation-independent decay ?
A: Degradation of messenger RNAs (mRNAs) plays a vital role in the modulation of expression of gene and…
Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification…
A: Protein purification is a process of isolation of specific protein from the complex protein mixture…
What is transformation efficiency and how is it calculated?
Transformants are the cells that are able to incorporate any modified foreign or artificial DNA. They also express the genes located on that piece of DNA that has been incorporated.
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