c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors binding around the Lrg1 gene in the four treatments. You use your data to generate the following figure. Orient yourself to the figure by finding the transcription start site on the x-axis. The size of the peak indicates the frequency of the transcription factor at that position. Lrg1 Lrg1 transcription Control . = 12 2. 3 Sucrose = 77 Sucrose + = 180 Hormone 4 Starvation = 2 -2,000 bp -1,000 bp +1 bp +1,000 bp What type of transcription factor might be present at position 2? What evidence i) from the figure supports your claim? (two sentences max) ii) What type of transcription factor might be present at position 3? What evidence from the figure supports your claim? (two sentences max) ii) Look at the transcription factors at position 4. Use evidence from the figure to explain the effect they are having on transcription of Lrg1. (two sentences max)
c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors binding around the Lrg1 gene in the four treatments. You use your data to generate the following figure. Orient yourself to the figure by finding the transcription start site on the x-axis. The size of the peak indicates the frequency of the transcription factor at that position. Lrg1 Lrg1 transcription Control . = 12 2. 3 Sucrose = 77 Sucrose + = 180 Hormone 4 Starvation = 2 -2,000 bp -1,000 bp +1 bp +1,000 bp What type of transcription factor might be present at position 2? What evidence i) from the figure supports your claim? (two sentences max) ii) What type of transcription factor might be present at position 3? What evidence from the figure supports your claim? (two sentences max) ii) Look at the transcription factors at position 4. Use evidence from the figure to explain the effect they are having on transcription of Lrg1. (two sentences max)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:iv)
What type of transcription factors might be present at position 6? What evidence
from the figure supports your claim? (two sentences max)
Based on the figure, propose a hypothesis for how addition of hormone affects
transcription of Lrg1, including the specific type of transcription factors triggered
by the hormone and what they do. (two sentences max)
v)
vi)
Explain why a transcription factor binds to some DNA sequences but not others.
(two sentences max)
Transcribed Image Text:c) You use chromatin immunoprecipitation to measure the location and amount of
transcription factors binding around the Lrg1 gene in the four treatments. You use your
data to generate the following figure. Orient yourself to the figure by finding the
transcription start site on the x-axis. The size of the peak indicates the frequency of the
transcription factor at that position.
Lrg1
Lrg1
transcription
Control u
= 12
2.
3
Sucrose
= 77
Sucrose +
= 180
Hormone
4
Starvation
= 2
-2,000 bp
-1,000 bp
+1 bp
+1,000 bp
i)
What type of transcription factor might be present at position 2? What evidence
from the figure supports your claim? (two sentences max)
What type of transcription factor might be present at position 3? What evidence
ii)
from the figure supports your claim? (two sentences max)
iii)
Look at the transcription factors at position 4. Use evidence from the figure to
explain the effect they are having on transcription of Lrg1. (two sentences max)
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