The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The sequence given is from the point where transcription starts to the point where transcription ends. 5'-АСТТCGАТАTGTСТААААТАСGATCGATCTGTGGGGCСТАGCТАGСТААССАGAGACGCTACCG-3' 3'-TGAAGCTАТАСAGATTTтТАTGСТАGСТAGACACCCCGGATCGATCGАТTGGTCTCTGCGATGGC-5. (e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to label the N and C termini of your molecule. (f) What is the nature of the mutation and how does it affect the protein?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 1P
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9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence
given is from the point where transcription starts (called "+1") to the point where transcription ends.
Transcription goes left to right.
5 -ACTTCGATATGTCTAAAATATCGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3'
3'-TGAAGCTATACAGATTTTATAGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5'
(a) Which strand (the upper or the lower) is used as the template in transcription?
(b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5'
and 3' ends of your molecule.
(c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to
label the N and C termini of your molecule.
The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The
nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The
sequence given is from the point where transcription starts to the point where transcription ends.
5 -ACTTCGATATGTCTAAAATACGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3'
3 -TGAAGCTATACAGATTTTATGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5'
(e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to
label the N and C termini of your molecule.
(f) What is the nature of the mutation and how does it affect the protein?
D Focus
F5
F7
F8
F10
F11
PriS
6
F12
%24
Transcribed Image Text:*00 II viruses. Unless you need to edit, it's safer to stay in Protected View. Enable Editing 1. 9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence given is from the point where transcription starts (called "+1") to the point where transcription ends. Transcription goes left to right. 5 -ACTTCGATATGTCTAAAATATCGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3' 3'-TGAAGCTATACAGATTTTATAGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5' (a) Which strand (the upper or the lower) is used as the template in transcription? (b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5' and 3' ends of your molecule. (c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to label the N and C termini of your molecule. The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The sequence given is from the point where transcription starts to the point where transcription ends. 5 -ACTTCGATATGTCTAAAATACGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3' 3 -TGAAGCTATACAGATTTTATGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5' (e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to label the N and C termini of your molecule. (f) What is the nature of the mutation and how does it affect the protein? D Focus F5 F7 F8 F10 F11 PriS 6 F12 %24
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