calculate the ALT concentration level in serum if the absorbance of the sample after 1 minute was 0.2 and after 2 minute was 0.25 and after 3 minute was 0.3 and the molar absorbance (ɛ) of NADH at 340 nm is 6300, the lightpath (I) is 1 cm, the total reaction volume (Vt) is 1.05 at 37°C, the sample volume (Vs) is 0.05mL ?

calculate the ALT concentration level in serum if the absorbance of the sample after 1 minute was 0.2 and after 2 minute was 0.25 and after 3 minute was 0.3 and the molar absorbance (ɛ) of NADH at 340 nm is 6300, the lightpath (I) is 1 cm, the total reaction volume (Vt) is 1.05 at 37°C, the sample volume (Vs) is 0.05mL ?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.13QAP: Copper(II) forms a 1:1 complex with the organic complexing agent R in acidic medium. The formation...

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The kinetics of hydrolysis of acetylsalicylic acid (aspirin) under certain conditions of temperature, pH, type of buffer and strength ionic, can be followed by multicomponent spectrophotometry. When performing a calculation of optical constants, the following data were obtained: Solution C. mg/100 mL A in λ (276nm) A in λ (290nm) Ac. acetylsalicylic ( AAS) 0.202 0.102 0.031 Ac. salicylic (AS) 0.198 0.158 0.101 Reaction: AAS + H2O → AS + CH3COOH In a kinetic study, after a certain time, 1 mL of the solution was taken from the reactor and diluted to 10 mL with water. distilled. Then when proceeding to the spectrophotometric measurement, the last solution presented the absorbances of 0.244 and 0.102 for the lengths of 276 and 290 nm respectively. What are the concentrations of ASA and AS in mg/L, at that instant in the reactor? Note: Acetic acid does not absorb at either wavelength.
Given the Information that Fe3++Y4FeYKi=1.01025Cu2++Y4CuY2Ki=6.31018 and the further information that, among the several reactants and products, only CuY2- absorbs radiationat 750 nm, describe how Cu(II) could be used as an indicator for the photometric titration of Fe(III) with H2Y2-. Reaction:+ Fe3++ H2Y2- FeY- + 2H+.
Copper(II) forms a 1:1 complex with the organic complexing agent R in acidic medium. The formation ofthe complex can be monitored by spectrophotometry at 480 nm. Use the following data collected under pseudo-first-order conditions to construct a calibration curve of rate versus concentration of R. Find the concentration of copper(II) in an unknown whose rate under the same conditions was 6.2 10- 3A s-1.
At 25oC the Ksp for PbCl2 is 1.6 × 10–5. Calculate Q for the following: 125.0 mL of 0.0900 M Pb(NO3)2 is mixed with 75.0 mL of 0.0200 M NaCl at 25oC.
4. determine the amount in mg of the amount of Ag present from ICP mass digested: 1.0821g/L dilution of 10ml stock solution to 100ml volumetric flask X=0.98 (intensity)
. A student collects the following data for the absorbance of a complex measured in 1.00 cmspectrophotometric cells. The molar absorptivity (ε) of the complex under investigation is 2.25×103L/mol·cm. Calculate the concentration of the complex in solution at each time of the reaction.Time (min)MeasuredAbsorbanceComplex Concentration(M)0.000 1.50010.000 1.31320.000 1.12530.000 0.93840.000 0.750
You have measured the blank for a determination of arsenic in food samples by hydride-generation atomic fluorescence spectrometry. The blank values are: 0.23 ppb, 0.14 ppb, 0.16 ppb, 0.28 ppb, 0.18 ppb, 0.09 ppb, 0.10 ppb, 0.20 ppb, 0.15 ppb, 0.21 ppb As. What is the LOD?
For a tungsten carbene (catalyst) binding to THF (ligand) at 25 oC in per-deuterotoluene solvent, the chemical shift of the unbound catalyst alpha hydrogen was determined to be 794.6 Hz and the peak for the fully bound catalyst alpha hydrogen was observed at 842.4 Hz. Furthermore, the binding constant (or formation constant) Kf was found to be 15.40 M-1. Given the following initial concentrations of catalyst and ligand, calculate the percent THF bound you would expect to see at 25 oC. Record your answer to the nearest tenths of a percent, as a number with no units (Ex. 3.4% ----> enter "3.4") Co = 1.000 M Lo = 0.1000 M
The Ksp of Al(OH)3 (MM: 78) is 1.90 xx 10^(-33). a) What is the solubility of Al(OH)3 in g/L?
What is the pathlength in centimeters when the molar absorptivity for a given absorbing species is 1.32 ¥ 103 L mol-1 cm-1, the concentration is 0.000923 M, and the absorbance is 0.493?
Ali has measured the oxidative stability of palm oil sample under accelerated storage condition at 60*C for 6 days The following data were recorded for the peroxide value and p anisidine value of the palm oil after 5 days of storage time: Sample weight 1.0030 g Concentration of NazSzO3 = 0.001 N Volume of NazSzO3 titrated for sample solution = 1.35 ml Volume of NazSzO3 titrated for blank solution 0.20 ml Absorbance for sample solution = 0.907 Absorbance for blank solution 0.203 Calculate: i) peroxide value ii) p-anisidine value iii) TOTOX value
Rate = -(Delta[RCl])/Delta t andln[RCl]t=-k*t+ln[RCl]0 < [R-Cl]-time equation Test tube A = 0.30 of 0.10M NaOH(aq), 6.70ml DI H2O & 1 drop of bromophenol bue Test Tube B = 3.00M of 0.10M R-CL in acetone. Mix together in 3 water baths Bath A Trial 1= 20C, 53.6s Trial 2= 20C, 49s for color to changeBath B Trial 1= 32C, 18s Trail 2= 32C, 20s Bath C Trial 1= 10C, 204s Trial B= 10C, 200sQ.6 Rearrange the Arrhenius equation into a linear form relating k and TQ.6a To determine Ea, a graph of (xaxis) ____ (in units of ___) vs. (yaxis) ____ needs to be made. This will yioeld a _____ line with a slope = ____ Q.6b enter values used in the table Q.6c Determine the exp. values of Ea with correct sig figs and units