Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein 

Table #2 Worktable for BCA Assay
Standard
Water (μl)
Tube #
1
2
3
4
5
6
7
8
9
0
125
325
175
325
325
325
400
400
BSA source (μl)
300 stock
375 stock
325 stock
175 of tube #2
325 of tube #3
325 of tube #5
325 of tube #6
100 of tube #7
0
Protein (ug)
Add the volumes listed in Table 2 to each tube. Add the water first and then the
appropriate standard. Pipette each of the protein samples directly into the water, do not
dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and
contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein (ug)).
Transcribed Image Text:Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).
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