Answered: How much of 10,000x SYBR safe would you…

Science

Biology

How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter1: Relative Value, Addition, And Subtraction Of Decimals

Section: Chapter Questions

Problem 39SST

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Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.

Question

How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x?

V1= 0.005mL

B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?

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