Calculate the final concentration of citrate buffer in a test tube containing the following components: 250 µl of 25 mM p-nitrophenol phosphate, 100 µL 0.5 mg.ml-1 acid phosphatase and 150 µl 100 mM citrate buffer pH 4.8.

Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.9: Spectrophotometry
Problem 3.1ACP
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Calculate the final concentration of citrate buffer in a test tube containing the following components: 250 µl of 25 mM p-nitrophenol phosphate, 100 µL 0.5 mg.ml-1 acid phosphatase and 150 µl 100 mM citrate buffer pH 4.8.

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