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- Estimate the Km and vmax from the data. [S] (M) Velocity (µM/min) 2.5 x 10-6 28 .00001 70 .00004 112 .0001 128 .002 139 .01 140 Km=.00001 vmax=140 Km=.002 vmax=112 Km=.01 vmax=140 Km=.00001 vmax=70https://m.youtube.com/watch?v=vM1SP346XBc&list=PLeJOSNLNZfHubfLdq0kOayASeUllMOGn4&index=4 I watched this the lecture video over and over and I am allowed to work with someone but I am having trouble with part B and I provided the YouTube link of the data or video attached to this labplease help this is my 4th time posting this and i havent gotten a correct answer. Please actually draw the graph out. A newly discovered enzyme, MBCase, cleaves carbon-carbon bonds of vicinal alcohol; The kinetic parameters were measured for 1.0 nM Of MBGase and determined to be a Km = 10 nM and a kcat = 100 s-1 a.) Sketch the graph out as a double-reciprocal plot (Lineweaver-Burke Plot) and label: -the axes (with units) -the point on the curve that can be used to determine Km -the point on the curve that can be used to determine Vmax
- (6.65x10^5)/(6.60x10^-6) (3.73x10^8) Please Calaculition. Only typed solutionPlease answer super fast and answer all questions and show calculations For the image attached For 1. a Mass of metal: Trial 1 is 35.0228 g Trial 2 is 35.0915 g Trial 3 is 34.0821 g Mass of water: Trial 1 is 20.0177 g Trial 2 is 20.0250 g Trial 3 is 20.0168 g For delta t of water: Trial 1 is 15.5 C Trial 2 is 15.7 C Trial 3 is 15.1 C For delta t of metal Trial 1 is 80.1 C Trial 2 is 80.2 C Trial 3 is 79.5 C For B my calculated Specific heat is: Trial 1 is 0.462 Trial 2 is 0.467 Trial 3 is 0.466calculate the flow rate in ml/hr . order 40meq/l at 10meq/hr in 1000ml in d5w supply 20meq/10ml
- If 5 µM enzyme was used to obtain the data in the summary plot below, V-max = ________________ µM sec-1 and Km = ____________ µM for this enzyme (Enter numeric values to the nearest integer; Do NOT write units.)Show all steps leading to the final answer po. Here’s a pdf file in accordance with the topic po: https://drive.google.com/file/d/1_FnDtXCrFKSol3RNWIG_9tNQ7IxgxD6t/view?usp=drivesdkDesign a procedure on how you will prepare 1.25 M table sugar (C12H22O11)solution and 1.25 M table salt (NaCl) solution diluted to 1 L water. Perform three trialseach set-up and get the average. Write/ encode your output in short bond paper withthe following parts:Title____________________I. Objectives:II. Materials:III. Procedure (including pictures/diagrams):IV. Data:V. Generalization: (Note: Perform this activity on a separate table/ area at home to ensure accuracyand precision of data. Be careful on handling the solution to avoid spillage.)
- A fly ash aerosol of monodisperse particles with a density of 2.0 g/mL and particle diameter of 10.0 mm has a concentration of 1000.0 mg/m3. Calculate the settling velocity in cm/s and the settling rate in g/m2-s. Please answer very soon will give rating surelyHelp with q=mcchange in temp ?Gas chromatography was used to analyse for residues of toxic solvents, including benzene and 1,2-dichloroethane (DCE), in a pharmaceutical preparation. The following conditions were used: Column: 30 m long capillary with polysiloxane stationary phaseCarrier gas: He at 35 cm s–1 constant flowOven program: 40 °C at t = 0, increasing at 10 °C min–1 to 240 °C at t = 20 min. Detector: flame ionisation (FID) Benzene eluted with retention time = 11.5 min, peak width = 0.26 min 1,2-dichloroethane (DCE) eluted with retention time = 11.8 min, peak width = 0.28 min (i) What are the strengths and limitations of the flame ionisation detector in this application, compared with other possible methods of detection? (ii) What is the resolution, RS, for the separation of benzene and DCE on this column? (iii) Suggest two ways by which the resolution of benzene and DCE might be improved. Explain your choices.